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Microbiology Module 1 '15 - '16 > Biochemicals > Flashcards

Flashcards in Biochemicals Deck (63):
1

What is the principle of the Methyl Red test?

If the organism can produce acid/neutral products via Glucose Fermentation.

2

What is the principle of the Voges-Proskauer test?

IF the product is Acetone from glucose fermentation

3

What is the principle of the Citrate test?

If the organism can use Citrate as its sole source of carbon.

4

What is the Principe of the Phenylalanine test?

Ability to deaminate Phenylalanine to Phenylpyruvic acid

5

What is the principle of the Indole test?

Spit indole from amino acid Tryprophane

6

What is the principle of the KIA test?

Ability to ferment lactose and/or glucose with/without Gas production as well as H2S

7

What is the principle of the motility test?

If an organism is motile (flagellated) or non-motile (non-flagellated)

8

What is the principle of the Urease test?

Able to split Urea via Urease

9

What is the principle of the Decarboxylase Dihydrolase test?

Presence of decarboxylase enzymes to break down Amino Acids to their Amine and CO2

10

What is the principle of the Arginine Dihyrolase test?

If an organic has arginine dihydrolase to break down arginine

11

What does a positive and negative mean in the methyl red test?

Positive used Mixed acid fermentation
Negative used Butylene Glycol

12

What fermentation do positive VP organisms use?

Butylene glycol fermentation

13

What is another name for the the phenylalanine test?

TDA

14

What are the two procedures of the Indole test?

Ehrlich and Kovacs

15

What is the advantage of one procedure over the other in the Indole test?

Ehrlich is more sensitive than Kovac

16

What is the Ehrlich procedure used for?

GNR and anarobes

17

What is the Kovacs procedure used for?

Enterobacteriaceae

18

What is the substrate and product of the KIA test?

Sodium Thiosulfate + H2S --> Black ppt. which is Ferrous Sulfide.

19

What is the difference in sugars among the Kia medias?

There is 10x more lactose than glucose and TSI has an additional 1% more sucrose

20

What can be added to the motility test, why? What is its disadvantage?

Tetrazolium Salts which help to visualize motility but can be inhibitory

21

What is the media used in urease? what is special about it?

Christens media and Proteus is strongly positive

22

What are two amino acids broken down in the Dacarb test and what are they broken down to?

Lysine to cadaverine and Ornithine to Putrescine

23

What is the substrate and reagent of the methyl red?

Glucose and methyl red

24

What is the substrate and reagent of the VP test?

Glucose; alpha naphthol AND KOH

25

What is the substrate and reagent of the Citrate test?

Citarte and NONE

26

What is the substrate and reagent of the Phenylalanine test?

Phenylalanine and FeCl3

27

What is the reagent used in the indole test?

P-dimethylaminobenzaldehyde-HCl

28

What is the substrate and reagent of the motility test?

None and NONE

29

What is the substrate and reagent of the Urease test?

Urea and NONE

30

What is the substrate and reagent of the decarboxylase dihyrolase test?

Lysine or ornithine and none

31

What is the substrate and reagent of the arginine dihyrolase test?

Argine and none

32

What is a positive and negative of the methyl red test?

Red is (+), Yellow is (-)

33

What is a positive and negative of the VP test?

Pink is (+), Yellow is (-)

34

What is a positive and negative of the Citrate test?

Blue is (+), Green is (-)

35

What is a positive and negative of the Phenylalanine test?

Dark Green is (+), Yellow is (-)

36

What is a positive and negative of the Indole test?

Red line in middle is (+) for Ehrich on the top is (+) for Kovac, Yellow is (-)

37

What is a positive and negative of the KIA test?

A/A is Glucose and lactose (Yellow/Yellow); K/A is only glucose (Pink/yellow) blacking with Pink top is assumed to have been glucose only and H2S (+) K/K is NONE (pink/pink)

38

What is a positive and negative of the Motility test?

Fanned/turbid is (+), single line is (-)

39

What is a positive and negative of the Urease tests?

Pink is (+), Yellow is (-)

40

What is a positive and negative of the Decarboxylase dihyrdolase test?

Purple/brown is (+), Yellow is (-)

41

What is a positive and negative of the Arginine dihydrolase test?

Purple/brown is (+), Yellow is (-)

42

What two tests should not be read early?

KIA and the decarboxylase

43

What is the principle of the carbohydrate 1% test?

Degrade carbohydrates to produce acid as well as gas or not

44

What is the principle of the Oxidation Fermentation test?

Utilization of glucose either fermentative, oxidative or neither

45

What is the principle of the Cytochrome Oxidase test?

Presence of oxidized cytochrome C

46

What is the principle of the Nitrate test?

Reduce nitrate to nitrite OR nitrate to N2 gas

47

What is the principle of the MUG test?

Looking for Beta-glucuronidase (in e.coli)

48

What is the broth and indicator in the 1% carbohydrate test?

Purple Broth base, bromcresol Purple indicator

49

What is the tube system called in OF, what is in it?

Hugh and Ceifson's, High Carbohydrate and Low peptones

50

How do you inoculate the oxidase test?

NEVER nichrome loops, wooden applicator

51

What is the medium with the nirate test?

Potassium nitrite

52

What is the full name of the MUG test?

4-methylumbelliferyl-Beta-P-Glucurdase

53

What is the substrate and reagent of the 1% carbohydrate test?

1!!! Carb and NONE

54

What is the substrate and reagent of the OF test?

Glucose and NONE

55

What is the substrate and reagent of the Cytochrome Oxidase test?

(filter paper) and 1% tetamethly-P-phenylenediaminedihyro chloride

56

What is the substrate and reagent of the Nitate test?

Nitrate and first (alpha naphylamine, sulfonic acid) then if neg, zinc dust

57

What is the substrate and reagent for the MUG test?

rapid test

58

What is a positive and negative of the 1% carbohydrate test?

Yellow (+) and Purple (-)

59

What is a positive and negative of the OF test?

Yellow in both = fermenter OR yellow in only oil= Oxidizer OR neither yellow (both green/red) = Asaccharolytic (non-oxidizer)

60

What is a positive and negative of the Oxidase test?

Purple/blue (+), no change (-)

61

What is a positive and negative of the Nitrate test?

Red (+) with first clear means you must add ZINC dust THEN RED (-) and CLEAR is (+)

62

What is a positive and negative of the MUG test?

Florescent (+) no florescence (-)

63

What is the main advantage of performing the Lead acetate test?

If is more sensitive for H2S than the KIA test is.