Neisseria and Moraxella Catarrhalis

Question 1

What are the characteristics and gram of Neisseria?

Answer 1

GNC(usually in pairs), aerobic, oxidase and catalase positive

Question 2

What are the characteristics and gram of Moraxella catarrhalis?

Answer 2

GNC(other Moraxella are rod shaped), graywhite colonies, “hockeypuck” colonies

Question 3

Where is Neisseria found?

Answer 3

Mucus membranes of man, normal oroparyngeal flora

Question 4

Where is Moraxella found?

Answer 4

normal oroparyngeal flora

Question 5

What are the growth requirements for pathogenic species?

Answer 5

fastidious

Question 6

What is the purpose of the candle jar

Answer 6

CO2 environment

Question 7

What is positive catarrhalis disk?

Answer 7

green

Question 8

What is ONPG testing for?

Answer 8

late utilization of glucose

Question 9

What are the 4 carbohydrates?

Answer 9

Glucose (dextrose), maltose, lactose and sucrose

Question 10

What is superoxol?

Answer 10

Same as catalase just use 30% instead of 3% H2O2

Question 11

G+, M-, L-, S-; What is this bacteria?

Answer 11

N. gonorrhoeae

Question 12

G+, M+, L-, S-; What is this bacteria?

Answer 12

N. meingitidis

Question 13

G+, M+, L+, S-; What is this bacteria?

Answer 13

N. lactamica

Question 14

G-, M-, L-, S-; What is this bacteria?

Answer 14

N. catarrhalis

Question 15

What are the pathogenic (group I) species?

Answer 15

N. gonorrhoeae and N. meningitidis

Question 16

What are the non-pathogenic species?

Answer 16

N. lacamica, M. catarrhalis, N. cinerea, N. mucosa, N.

sicca, N. subflava, N. flavescens, M. catarrhalis and others

Question 17

What media will pathogenic species grow on?

Answer 17

Selective

Question 18

What media will non-pathogenic species grow on?

Answer 18

not selective but some can

Question 19

Who is GNID on and why not both sexes?

Answer 19

Males it is diagnostic, females cant have it due to normal flora

Question 20

What does bacteria in joint fluid mean?

Answer 20

significant, should be sterile

Question 21

What is CSF cultured on?

Answer 21

Chocolate

Question 22

What are disease associated caused with N. gonorrhoeae?

Answer 22

gonorrhea,eye infections, septicemia, pharyngitis, arthritis

Question 23

What are disease associated caused with N. meningitides?

Answer 23

maybe normal respiratory flora, meningitis,

septicemia, DIC, Waterhouse Friderichsen syndrome

Question 24

How is N. gonorrhoeae diagnosis made?

Answer 24

Positive GNID

Question 25

What is the proper specimen collention and transport for gonorrhea?

Answer 25

Dacron or rayon swabs, urethra in males and endocervix in females

Question 26

Resistance and treatment trends with N. gonorrhoeae

Answer 26

Widespread, multidrug therapy, also treat co-infection with Chlamydia

Question 27

What are the advantages and disadvantages of conventional and molecular testing for N. gonorrhoeae?

Answer 27

Advan-rapid, perform on clinical specimen, live organisms not required, chlamydia and tricomanas tested at same time. DISADVAN- not admissible in court, no live organism for susceptibility, expensive

Question 28

How do most individuals become infected with N. meningitides?

Answer 28

Close contact (young adults) dorms, barracks

Question 29

What are the distinguishing characteristics of waterhouse-friderichsen syndrome?

Answer 29

hemorrhage into adrenal glands, shock, rapid death

Question 30

Why is it critical to work under a hood with N, menigitidis and not N. gonorrhoeae?

Answer 30

pilli, Gonorrhoeae loses them and can not infect, Menigitidis can

Question 31

What is the recommended treatment for at risk N. meningitis people?

Answer 31

Rifampin

Question 32

Why are those in the US not fully protected against N. meningitids?

Answer 32

type B vaccine just became avaliable

Question 33

Gray white colonies, hockey puck, rowth on SBA and CHOC, no acid production from carbs, DNase +, butyrase esterase +

Answer 33

Moraxella catarrhalis

Question 34

What organism is misidentifies as N. gonorrhoeae using carbs?

Answer 34

N. cinerae

Question 35

Compare and contrast N. cinerae and N. gonorrhoeae

Answer 35

Look similar, N. cinerae is not pathogenic

Question 36

What are characteristics of less common Neisseria?

Answer 36

Yellow/greenish, dry and wrinkled colonies, N. sicca is G+, M+, L-, S+