Haemophilus and Bordetella Flashcards
(39 cards)
What are the gram characteristics of Haemophilus?
Tiny GNR (can look coccobacillary), can be long filamentous rods
What are the characteristics of Haemophilus?
Very fastidious, Capnophiliic, mousy smell, oxidase AND catalase positive most are opportunistic and adapted the respiratory tract. Require growth factors (i.e. X, V or Both)
What is X factor, where is it found?
Hemin. It is found in chocolate and is HEAT STABLE
What is V factor, where is it found?
NAD, in RBS but inhibited by enzymes in humans. Also present in yeast extract (chocolate agar)
What species require V factor?
All BUT Aggregaatibacter aphrophilus and Haemphilus ducreyi
What are the two methods for identifying X and V factor?
Staph streak or disks
What factors are in T-Soy plates?
None
What factors are in SBA
Only X
What factors are released by stapylcoccus aureus?
V
What factors are in chocolate agar?
X and V
What factors are in Rabbit blood Agar?
X and V
What factors are in Fildes Enrichment agar?
X and V
What characteristic does a rabbit blood plate help to identify? What part of the bacteria name indicates this?
BETA hemolysis, haemolyticus
What is an alternative form of determining X factor? What does it eliminate? What is its basis?
Porphyrin, Eliminates Hemin carryover and it fluroeses
What is the clinical significance of H. flu?
It is a secondary invader with Pneumonia and in noninvasive NTHi (nontypable)
What is the Primary virulence factor with H. flu?
The Capsule
What strain does the H. flu vaccine fight against?
Type B, so obviously if you get an infection and have had the vaccine, it is prob. not type B
What are the plate characteristics of H. influenzae?
Smooth colonies on choclate, requires both X and V and is non-hemolytic
What does para mean when it is with the bacteria name?
It requires only V factor
What are 3 bacteria that can provide V factor?
S. aureus, S. pneumoniae and Neisseria
If an organism grow on chocolate, what can you say about its factors?
It MAY require X and V
If an organism satellites around S. Aureus on SBA, what can you say about its factors?
It requires V and MAY require X
If an organism saellites around S. Aureus on T-soy, What can you say about its factors?
It requires only V
What ca you say about an organism that grows on SBA concerning its factors?
Does not require V
