Biochemistry

Question 1

Amanita phalloides inhibits

Answer 1

RNA polymerase

Question 2

Enzymes with ZINC as co factor

Answer 2

Carbonic anhydrase
Carboxy peptidase A and B
Cytosolic SOD
Alcohol dehydrogenase
Lactate dehydrogenase
ALA dehydratase
Adenosine deaminase
Alkaline phosphatase

Question 3

Daily requirement of magnesium

Answer 3

400mg/day

Question 4

At what blood glucose levels, does insulin begin to rise?

Answer 4

> 70mg/dL or 3.9mmol/L

Question 5

Enzymes with magnesium as co factor

Answer 5

Kinase
Phosphatase
Mutase
Enolase

Question 7

Enzyme deficiency in von girkes

Answer 7

Glucose 6 phosphatase

Question 8

Enzyme deficiency in type 1b

Answer 8

Transporter of G-6PO4

Question 9

Enzyme deficiency in Cori’s disease

Answer 9

Debranching enzyme

Question 10

Enzyme deficiency in Anderson’s disease

Answer 10

Branching enzyme

Question 13

Enzyme deficiency in Her’s disease

Answer 13

Hepatic Glycogen phosphorylase

Question 14

Enzyme deficiency in Pompe’s disease

Answer 14

Acid maltase

Question 15

Enzyme deficiency in Mc ardles disease

Answer 15

Muscle glycogen phosphorylase

Question 17

Enzyme deficiency in Tarui’s disease

Answer 17

Erythrocyte/ Muscle PFK-1

Question 18

Michelis menten equation

Answer 18

V1= Vmax * [S] / Km + [ S]

Question 19

Kcat= Vmax/ total enzyme conc

Question 20

Catalytic efficiency of an enzyme=

Answer 20

Kcat/Km

Question 21

Examples of uncompetitive inhibition

Answer 21

Phenylalanine inhibits placental ALP

Question 22

What is an aldonic acid

Answer 22

Oxidation at aldehyde group

Question 23

D and L isomerism

Question 24

What is a uronic acid

Answer 24

Oxidation at 6th carbon atom

Question 25

What is a saccharic acid

Answer 25

Oxidation at both aldehyde group and 6th C atom

Question 26

Formula for hyaluronic acid

Answer 26

N acetyl glucosamine+ glucuronic acid

Question 27

Formula for keratan sulphate

Answer 27

N acetyl glucosamine+ galactose

Question 28

Formula for chondroitin sulphate

Answer 28

N acetyl galactosamine + glucuronic acid

Question 29

Formula for dermatan sulphate

Answer 29

N acetyl galactosamine+ iduronic acid

Question 30

Formula for heparan sulphate

Answer 30

Glucosamine + glucuronic acid

Question 31

Formula for heparin

Answer 31

Glucosamine+ iduronic acid

Question 32

Common features of mucopolysaccharidosis

Answer 32

Gargoylic faces- vertical facies Frontal bossing Depressed basal bridge Gingival hypertrophy Large tongue Corneal clouding Mental retardation Visceralomegaly Umbilical hernia

Question 33

Location of glycolysis

Answer 33

Cytoplasm

Question 34

Location of link reaction

Answer 34

Mitochondria

Question 35

Location of glycogenesis

Answer 35

Cytoplasm

Question 36

What is cahill's cycle

Answer 36

Glucose alanine shuttle

Question 37

What is Cori's cycle

Answer 37

Glucose Lactate shuttle

Question 38

ATP required to form 1 glucose from 2 lactate

Answer 38

6 ATP

Question 40

Enzyme deficiency in classic galactosemia

Answer 40

Galactose 1 P uridyl transferase

Question 41

Enzyme deficiency in Hereditary fructose intolerance

Answer 41

Aldolase B

Question 42

Specific test for galactosuria

Answer 42

Mucic acid test

Question 43

Specific test for fructosuria

Answer 43

Seliwanoff test/ Rapid furfural test

Question 44

Phospholipids with nitrogenous base

Answer 44

Lecithin Phosphatidyl serine Cephalin

Question 45

Phospholipids with non nitrogenous base

Answer 45

Cardiolipin Phosphatidyl inositol Phosphatidyl glycerol

Question 46

Histology feature in mucopolysaccharidosis

Answer 46

Reilly body inclusions in leucocytes

Question 47

Anomerism

Question 48

Epimerism

Question 49

Examples of suicide inhibition

Answer 49

Allopurinol inhibits xanthine oxidase Aspirin inhibits COX DfMO inhibits ornithine decarboxylase