Flashcards in Biotechnology Deck (17)
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1
Plasmid
DNA that can provide a selective advantage
often used as a "cloning vector"
2
Restricting Enzymes
bacterial enzymes that cut wherever a specific nucleotide sequence occurs
3
DNA ligase
binds together the "sticky ends" of DNA strands
4
Recombinant DNA
composed of DNA from two or more organisms
5
Selective Markers
can be used to see which cells have been transformed
ex) antibiotic resistance, color change
6
humilin
genetically engineered human insulin
7
What is used to produce humilin?
genetically altered E. Coli
8
human immune systems developed antibodies against what? what was the result?
bovine and porcine insulin
inflammatory response at injection site
9
BONUS: who is Dr. Paul Berg
widely regarded as a pioneer in genetic engineering
won the Nobel Prize in 1980
10
Bt Toxin
Bacillus thuringiensis bacteria produces a protein toxic only to insect larvae
11
what is the problem with Bt corn?
it is not approved by the FDA for human consumption
12
StarLink Corn
contains a protein known as Cry9C (natural Bt toxin)
13
Cry9C
protein that is not easily digested so it could possibly be an allergen in humans
14
Golden Rice
gene for beta carotene production was transferred from a daffodil into a rice plant
15
beta carotine is the precursor to what?
production of Vitamin A
16
Short Tandem Repeats
multiple copies of a short DNA sequence that follows one another along a chromosome
number and distribution are unique among all individuals
17
