Biotechnology Flashcards Preview

BMB > Biotechnology > Flashcards

Flashcards in Biotechnology Deck (17)
Loading flashcards...
1

Plasmid

DNA that can provide a selective advantage
often used as a "cloning vector"

2

Restricting Enzymes

bacterial enzymes that cut wherever a specific nucleotide sequence occurs

3

DNA ligase

binds together the "sticky ends" of DNA strands

4

Recombinant DNA

composed of DNA from two or more organisms

5

Selective Markers

can be used to see which cells have been transformed

ex) antibiotic resistance, color change

6

humilin

genetically engineered human insulin

7

What is used to produce humilin?

genetically altered E. Coli

8

human immune systems developed antibodies against what? what was the result?

bovine and porcine insulin
inflammatory response at injection site

9

BONUS: who is Dr. Paul Berg

widely regarded as a pioneer in genetic engineering
won the Nobel Prize in 1980

10

Bt Toxin

Bacillus thuringiensis bacteria produces a protein toxic only to insect larvae

11

what is the problem with Bt corn?

it is not approved by the FDA for human consumption

12

StarLink Corn

contains a protein known as Cry9C (natural Bt toxin)

13

Cry9C

protein that is not easily digested so it could possibly be an allergen in humans

14

Golden Rice

gene for beta carotene production was transferred from a daffodil into a rice plant

15

beta carotine is the precursor to what?

production of Vitamin A

16

Short Tandem Repeats

multiple copies of a short DNA sequence that follows one another along a chromosome

number and distribution are unique among all individuals

17

Gel Electrophoresis

reveals the DNA fingerprint/ sequence of short tandem repeats

electric field pulls all DNA fragments through a semi-solid gel according to their length as they migrate