Biotechnology Flashcards
(5 cards)
1
Q
Gel electrophoresis
A
Electrical current moves DNA fragments through gel. Smaller fragments move faster. Longer fragments have more repeating segments. In forensics, repeated with different restriction enzymes.
2
Q
PCR
A
Copy and amplify DNA. Polymerase 3.
3
Q
Recombinant DNA
A
Joining of two different DNA molecules. Plasmid technique.
4
Q
Cloning
A
Dolly. Reproductive cloning, produces new individual.
Therapeutic cloning.
5
Q
Restriction enzymes
A
Recognize base pair sequences called restriction sites and cut DNA there. Cuts are asymmetrical and produce sticky ends. These connect to complements.
