Blah 3 Flashcards
(8 cards)
1
Q
cDNA phages
A
Sub cloning
Zap2 xc1 solr/xlolr exassist cole1 ori p bluescriot
Triplx crelox loxxp sites
2
Q
Maxam Gilbert
Sanger
A
Pipe ridine
Didiixyl if 3’ hydroxyl removed
3
Q
Primer extension
Nucleases protection assay
A
RNA primer cDNA run parallel 1bp
Probe rnaset1 dnase1 cuts sals see product
4
Q
Conserved intron
A
3’ag 5’ gr
5
Q
Run off
Reporter
In situ
A
Label 32 p
Histology cal preparations
6
Q
RNA protection
A
Liquid hybridisation RNA digest probes detect RNA transcript
7
Q
Micro arrays
Hrts Illumina
A
Global levels in diff conditions
Algorithms and look at infection time s
8
Q
2!d strand
A
Hairpin hydroxide then s1 Rnase then ligase Homopolymer transferase not1 asc1 Full cDNA polyphosphatase then tobacco acid phosphatase not1 asc1 Adaptora methylated ctps then xho1
