Stuff to learn Flashcards
(19 cards)
Where do type 1 REs cleavve
far away from recognition site about 1000bp
names five type 2 Res
ecor1 hae111 pstl Hindll Kpnl
what does Bal31 require to functions
Ca2+
What are the aplications of pcr
forensics diagnosis
what is used to shear ends and cut into fragements in making genomic libraries
sau3A1
what is another genomic lobrary vector and insert size
plasmid 15kb
what is an example of a lambda phage vector for lib
lambda EMBL3
what do cohesive ends allow
packaging in virus particles
megabase
gigbase
mega = million gia = billion
what is an epitote
part of antigen recognised by the immune system
what is a illumina sbs called
solexia
describe the two types of fluroescene real time pcrs
probe flourescent add fluroescent probe that bidns in the middle of the two primers to ss template
probe gets degraded by dnap causeing speration from fluresecent reporter
seperation causes more fluoresecne.
amoutn can be be determined quantitatively
- use syber greent hat ony intercalates in dsDNA and fluoresces there
micro injection with mice what do you inject into
male pronuclease of fertiled egg
do adeno viruses have an enveleop
no
what is adenovirus seq flanked by
ITR
inverted terminal repeats
describe x-skid case study
used a retrovirus to treat it high success but some got leukemia reported vector interrgrated into LMO-2 gene causing leukemia.
= insertional mutagenesis
what genes do retrovirses have
gag
pol - integrase
env - envelope
what is the best kind of AAV used and why
AAV2
inserts into non vital part of chromosome 19
whatdisease study used AAV
parkinsons and prostate study
