Blah

Question 1

Cloning

cDNA

Answer 1

RT alkali and high temp
C tail transferase
Pbr322

Question 2

Slic
Extension
Gateway

Answer 2

3’ exonucleaae t4 polymerase overlap
Chinaerix phusion and 2 5’ nicks dpn1 digest
Site specific recomb LR–> BP lytic INt IHF Xis

Question 3

Cloning double blunt golden gate topoisomerase

Answer 3

Double bamh1 bgl2
Blunt ecor4 hpa1
Golden gat Bsa1 bbsa1 bsmB1
Ccctt vaccinia virus