Blah2 Flashcards

(3 cards)

1
Q

Phage
Cosmid
Yac
DNA lib

A

Bamh1 to remove uneccessafy for rep ligase cos sites
Digest ligase amp
Human digest ecorv and bamh1

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2
Q

Colony lift

Chromosomal walking

A

Nitocelluse nylon
Noah and protease uv 80
Sequential hybridisation

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3
Q

Extraction in DNA lib

A

Phenol sau3a1

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