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Polypeptide capsule

B. anthracis

All other are polysaccharide

1

Bacteria with unusual cell walls

Mycoplasma - Sterols & no cell wall

Mycobacteria - Mycolic acid & high lipid content

2

What are the spirochetes?

Borrelia
Leptospira
Treponema

3

Giemsa stained bugs

"Certain Bugs Really Try my Patience"

Chlamydia
Borrelia
Rickettsiae
Trypanosomes
Plasmodium

4

PAS Stain bugs

Stains glycogen

Tropheryma whipplei (whipple's disease)

5

Zeihl-Neelsen bugs

Acid-fast organisms

Nocardia
Mycobacterium

6

India ink bugs

Cryptococcus neoformans

7

Silver stain bugs

Fungi
Legionella
H. pylori

8

H. flu culture requirements

Chocolate agar (Factor V & X)

9

Neisseria (both) culture requirements

Thayer-Martin (VPN: Vanc, Polymixin, Nystatin) media

To inhibit all others

10

Bordetella pertussis culture requirements

Bordet-Gengou (potato) agar

Bordet for Bordetella

11

Corynebacterium diphtheriae culture requirements

Tellurite Plate, Loffler's media

Teller she's right, laugh at her after

12

MTB culture requirements

Lowenstein-Jensen agar

13

Mycoplasma pneumoniae culture requirements

Eaton's agar

14

Lactose+ enterics culture requirements

MacConkey's agar

(shows pink colonies)

15

Legionella culture requirements

Charcoal yeast extract with cysteine & iron

16

Fungi culture requirements

Sabouraud's Agar

"Sab's a fun-guy"

17

Obligate aerobes

"Nagging Pests Must Breathe"

Nocardia
Pseudomonas
MTB
Bacillus

18

Obligate anaerobes

Clostridium
Bacteroides

Generally foul-smelling, produce gas in tissue, and resistant to amin(O2)glycosides

19

Obligate intracellular bacteria

Stay inside when it's Really Cold:
Rickettsia
Chlamydia

They can't make their own ATP.

20

Facultative intracellular bacteria

"Some Nasty Bugs May Live FacultativeLY"

Salmonella
Neisseria
Brucella
Mycobacterium
Listeria
Francisella
Legionella
Yersinia pestis

21

Test for encapsulated bacteria

Quellung reaction (anti-capsule antisera)

If serum is added --> qapsular swellung (quellung)

22

Encapsulated bacteria

SHiNE SKiS

Strep pneumo
H. flu
Neisseria meningitidis
E. coli
Salmonella
Klebsiella
Strep B (GBS)

23

Catalase positive organisms

"You need PLACESS for your cats"

Pseudomonas
Listeria
Aspergillus
Candida
E. coli
Staph aureus
Serratia

24

Protein-conjugated vaccines

Prevnar (pneumococcal)
H. flu type B
Meningococcal

25

Urease positive organisms

K PUNCHES

Klebsiella
Proteus
Ureaplasma
Nocardia
Cryptococcus (fungi)
H. pylori
Epidermidis
Saprophyticus

26

Pigment producing bacteria:
Red
Green/Blue
Yellow

Red = Serratia
Green/Blue = Pseudomonas
Yellow = either Actinomyces israelii or Staph aureus

27

IgA protease containing bacteria

SHiN

Strep pneumo
H. influenza (Type B)
Neisseria

They can all transform DNA and all have an IgA protease (to colonize respiratory epithelium)

28

Transforming bacteria

SHiN

Strep pneumo
H. influenza (Type B)
Neisseria

They also have IgA protease

29

Group A Strep virulence factor

M protein

Prevents phagocytosis