Cell Mediated Cytotoxicity

Question 0

What are the five effector molecules produced by both NKC’s and CTL’s?

Answer 0

FASL, Granzymes, Perforins, IFNg, TNF

Question 1

What are two ways NK cells can determine which cell to kill?

Answer 1

1. Missing self-antigen and toxic activating ligand

2. Stress induced ligand and toxic activating ligand

Question 2

What three signals are needed for a CTL to mature?

Answer 2

1. Antigen specific recognition
2. CD28/B7 interaction
3. IL-2 signal from CD4

Question 3

These cells present CD45RA

Answer 3

Naive CTL-P

Question 4

These cells require a high level of IL-2 to proliferate.

Answer 4

Mature CTL

Question 5

These cells produce high levels of CD45RO.

Answer 5

Mature T cells.

Question 6

These cells produce low levels of CD2 and LFA-1.

Answer 6

Naive CTL-P

Question 7

These cells require very low levels of IL2 to proliferate

Answer 7

Memory CTL

Question 8

Which CTL complex recognizes MHC I?

Answer 8

TCR/CD3

Question 9

Antigen activation converts _____ to a higher affinity state for better binding.

Answer 9

LFA-1

Question 10

Granzyme molecules activate apoptosis by…

Answer 10

Cleaving caspases

Question 11

Fas ligand activates apoptosis by…

Answer 11

Cleaving caspases

Question 12

CTL’s can also kill by _____ secretion.

Answer 12

TNF

Question 13

Caspase-9 becomes activated once binding to mitochondrial ______ and cytoplasmic ______.

Answer 13

Cytochrome C and Apaf-1

Question 14

The Caspase-3 product gets activated by ______ and ______.

Answer 14

Caspase 8 and the Caspase 9 complex

Question 16

IFNg promotes stimulation of _____ from macrophages and dendritic cells.

Answer 16

IL-12

Question 17

NK cells are stimulated by what five cytokines?

Answer 17

IFN a, b, g, IL-15 and TNFa

Question 18

What transcription factors are important in forming IFNb?

Answer 18

IRF3, NF-kB

Question 19

What are the important transcription factors for IFNa?

Answer 19

IRF7 and NF-kB

Question 20

NK cells produce this receptor for Ig constant domains.

Answer 20

FcgRIII

Question 21

What is the key difference between what NK and CTL’s kill?

Answer 21

NK’s don’t need antigen.

Question 22

Inhibitory receptors on the NK cell have this domain on the inside.

Answer 22

ITIM

Question 23

Activating receptors of NK cells have this domain on the intracellular side.

Answer 23

K

Question 24

KIR (killer immunoglobulin receptors) are either long or short according to what?

Answer 24

Long: Inactivating
Short: activating

Question 25

What is NKG2A?

Answer 25

An inhibitory C-type lectin.

Question 26

What does NKG2A bind to?

Answer 26

HLA-E

Question 27

At what stage do NK cells produce KIR?

Answer 27

VERY mature.

Question 28

What receptor is meant to be a fallback for inhibition of NK induced death?

Answer 28

HLA-E

Question 29

What 5 cells are associated with Antibody Dependent Cell Mediated Cytotoxicity?

Answer 29

``` NK Macrophages Monocytes Neutrophils Eosinophils ```

Question 30

Of the cells associated with ADCC, which release TNF?

Answer 30

NK Monocytes Macrophages

Question 31

Of the cells associated with ADCC, which release perforin?

Answer 31

NK and eosinophils

Question 46

NK cells produce this important cytokine.

Answer 46

IFNg