Ch 6 RPR & Mono

Question 1

Rapid Plasma Reagin is a ________ test for serologic detection of the antibody Reagin

Answer 1

non-treponemal

Question 2

Reagin is a phospholipid produced in patients infected with

Answer 2

Treponema pallidum

Question 3

Causative agent of syphilis and acts like an antigen

Answer 3

Treponema pallidum

Question 4

Reagin is found in the _____ of patients with syphilis

Answer 4

plasma/serum

Question 5

Vehicle or antigen

Answer 5

Carbon charcoal particle

Question 6

Reactive result

Answer 6

Flocculation

Question 7

FTA-ABS

Answer 7

Fluorescent treponemal antibody-absorbed test

Question 8

Confirmatory test for RPR

Answer 8

FTA-ABS

Question 9

FTA-ABS

Fixed to a slide and patient’s serum is added

Answer 9

Dead T. pallidum

Question 10

FTA-ABS

Ultraviolet microscope is used, Positive result

Answer 10

Fluoresce

Question 11

RPR

Tube top color used for serum

Answer 11

Red/SST

Question 12

RPR

Tube top color for plasma

Answer 12

Purple

Question 13

After centrifugation, serum should be:

Answer 13

Clear and non-hemolyzed

Question 14

RPR Card Test kit

Answer 14

RPR Card antigen suspension

Plastic disposable bottle

20 Gauge, galvanized needles, blunt

Test cards

Pipettes/stirrers

Test Controls

1 mL TB Syringe

Distilled water

Mechanical rotator

Question 15

RPR

Mix the antigen by:

Answer 15

Shaking vigorously for 10-15 seconds

Question 16

RPR

Dispense number of drops

Answer 16

1 drop

Question 17

RPR

Number of drops for the antigen to each specimen

Answer 17

1

Question 18

RPR test card should be on rotator for:

Answer 18

8 minutes at 100 rpm

Question 19

RPR

Read the reaction time after:

Answer 19

8 minutes on rotator

Question 20

Helps differentiate non-reactive from weakly reactive results

Answer 20

Tilting the card by hand (3 to 4 to-and-fro motion)

Question 21

Read macroscopically under:

Answer 21

high intensity lamp or strong daylight

Question 22

RPR

Report results as:

Answer 22

Reactive or Non-reactive

Question 23

Reactive RPR tests shows flocculation ranging from:

Answer 23

Slight but definite

Minimum to moderate

Marked and intense

Question 24

RPR tests must be confirmed with:

Answer 24

FTA-ABS

Question 25

How often are controls ran with each batch of Rapid Plasma Reagin tests?

Answer 25

Always

Question 26

Ensures the accuracy and reliability of test results

Answer 26

Quality Control

Question 27

Quality Control starts with:

Answer 27

Proper collection and handling of specimen Assaying control reagents Checking instrument performance

Question 28

Two sections of Quality Control

Answer 28

Equipment quality control Reagent quality control

Question 29

Calibration of the mechanical rotator

Answer 29

100 rpm

Question 30

Calibration of the antigen needle

Answer 30

60 drops per 1 mL

Question 31

Controls used in RPR

Answer 31

Reactive Weak-reactive Non-reactive

Question 32

Control reagents are treated as patients and ran every time an:

Answer 32

Unknown sample is tested

Question 33

Mononucleosis is caused by

Answer 33

Epstein-Barr virus

Question 34

Mono is typically seen in:

Answer 34

Young adults and children <5 years old

Question 35

Symptoms of mono

Answer 35

Fever Malaise Lethargy Sore throat w/ exudates Enlarged lymph nodes of neck Mild hepatitis Enlarged spleen Blotchy skin rash

Question 36

Causes the formation of heterophile antibody

Answer 36

Increased and abnormal forms of lymphocytes and monocytes

Question 37

An antibody that is structurally similar to the antibody that is specifically produced as a response to antigen stimulation

Answer 37

Heterophile antibody

Question 38

Mono blood samples used

Answer 38

Serum or plasma

Question 39

Monospot Latex Test Kit

Answer 39

Latex Reagent suspension Pipettes/stirrers Test Slides Positive and Negative Control

Question 40

Used to sediment cellular elements

Answer 40

Centrifuge

Question 41

Mono Test the specimen within 24 hours of blood collection if stored:

Answer 41

2-8 degrees Celsius

Question 42

Allow the Latex Reagent and controls to reach:

Answer 42

Room Temperature (20 to 30 degrees C)

Question 43

Shake the Latex Reagent:

Answer 43

Gently

Question 44

Add ___ of Latex Reagent next to the drop of sample

Answer 44

1 drop

Question 45

Mono Gently rotate the slide ___ minutes manually

Answer 45

3

Question 46

Mono Use a rotatory shaker set at:

Answer 46

60-100 rpm

Question 47

Mono: Look for the presence of:

Answer 47

Agglutination

Question 48

Mono Report results as either:

Answer 48

Positive or Negative

Question 49

Mono A negative result with demonstrate:

Answer 49

Smooth, homogenous solution with no agglutination

Question 50

Number of controls for monospot

Answer 50

2 Positive and Negative