CHAPTER 1 (PLASMID EXTRACTION) Flashcards
(5 cards)
1
Q
what is alkaline lysis in plasmid extraction
A
a method that uses a high pH and detergent to lyse bacteria, denature DNA, and separate small plasmid DNA from big chromosomal DNA.
2
Q
what does the alkaline condition do to linear DNA and plasmid
A
denature them
3
Q
how to recover plasmid after getting denatured
A
adding sodium or potassium acetate to neutralise the pH
4
Q
why neutralising the pH can renature the plasmid
A
cus of its structure that is small, supercoiled and circular
5
Q
what are other things inside the bacterial cell containing plasmid?
A
genomic DNA.
which why we extract the plasmid the way that we did.
