CHAPTER 2 (INTRO TO PCR: DNA CLONING)

Question 1

whats dna cloning?

Answer 1

making many identical copies of a specific DNA fragment by inserting it into a host organism (like bacteria) using a vector (like a plasmid).

In short: copying DNA by growing it in cells.

Question 2

cloned genes are useful for

Answer 2

1. producing a protein product
2. making copies of a particular gene

Question 3

what are the basics u need for DNA cloning?

Answer 3

1. source of dna - to be cloned
2. choice of vectors
3. restriction enzymes
4. dna ligase
5. host cell