Chapter 10.1 DNA Flashcards

1
Q

who investigated bacteriophages?

A

Hershey & Chase

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2
Q

bacteriophages are

A

viruses that infect bacteria

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3
Q

bacteriophages are composed of

A

only DNA and protein

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4
Q

in the experiment virus DNA were labeled with

A

radioactive phosphorus

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5
Q

in the experiment virus protein was labeled with

A

radioactive sulfur

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6
Q

viruses do not have a plasma membrane, instead a _____ is present

A

capsid

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7
Q

what was the conclusion of the experiment carried out by Hershey and Chase?

A

DNA is the genetic material

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8
Q

DNA is a

A

nucleic acid composed of nucleotides

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9
Q

what are the four characteristics of DNA structure?

A
  1. 5-carbon sugar
  2. phosphate group
  3. nitrogenous bases
  4. hydroxyl group
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10
Q

the five carbon sugar in DNA is called?

A

deoxyribose

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11
Q

in DNA where is the phosphate group located on the sugar?

A

at the 5’ carbon

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12
Q

in DNA where is the hydroxyl group located in the sugar?

A

at the 3’ carbon

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13
Q

what are the 4 nitrogenous bases of DNA

A
  1. adenine
  2. thymine
  3. cytosine
  4. guanine
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14
Q

what is a phosphodiester bond?

A

a bond between adjacent nucleotides

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15
Q

a phosphodiester bond is formed between the

A

phosphate group of one nucleotide and the 3’ (-OH) of the next nucleotide

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16
Q

the chain of nucleotides has what type of orientation

A

5’-3’ orientation

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17
Q

Erwin Chargaff determined that

A

there are always equal proportions of purines and pyrimidines

18
Q

Rosalind Franklin is known for

A

studies where she identified the 3-D structure of DNA

19
Q

James Watson and Francis Crick proposed

A

a double helix structure to DNA

20
Q

the bonds holding the phosphate and sugar groups are

A

covalent bonds

21
Q

the bonds holding the nitrogenous bases are

A

hydrogen bonds

22
Q

what 3 things are needed for DNA replication?

A
  1. something to copy
  2. something to do the copying
  3. building blocks to make the copy
23
Q

what is known as the “something to copy”?

A

parental DNA molecule

24
Q

what is known as the “something to do the copying”?

25
what is known as the "building blocks to make the copy"?
nucleotide bases
26
the DNA polymerase reads in what direction?
3' -> 5'
27
DNA is synthesized in what direction?
5' -> 3'
28
DNA replication includes what 3 things?
1. initiation 2. elongation 3. termination
29
the primer required to start DNA replication is synthesized by what?
primase
30
what is the primer needed to start DNA replication?
a short single strand of RNA
31
DNA helicase...
uses energy from ATP hydrolysis to unwind the DNA
32
what keeps the DNA strands from coming back together after they have been unzipped?
single-stranded binding proteins
33
which stand is pointed in the "right" direction?
the leading strand
34
which strand is pointed in the "wrong" direction?
the lagging strand
35
what are Okazaki fragments?
discontinuous strands of replicated DNA
36
what replaces the primers after DNA replication?
DNA polymerase
37
DNA ligase forms the...
final phosphodiester linkage between fragments
38
DNA repair mechanisms include...
1. proofreading 2. mismatch repair 3. excision repair
39
explain the proofreading mechanism of DNA repair
DNA polymerase adds a nucleotide to a growing strand
40
explain the mismatch repair mechanism of DNA repair
replaces a whole section of DNA even if just one base has been mismatched
41
explain the excision repair mechanism of DNA repair
enzymes scan for mispaired bases, chemically modifies bases, or extra bases