Chapter 11 Flashcards

1
Q

nucleotides

A

building blocks of nucleic acids

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2
Q

ATP

A

nucleotide

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3
Q

purines

A

adenine, guanine

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4
Q

pyrimidines

A

cytosine, thymine, uracil

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5
Q

How are purines and pyrimidines attached to C1’ of the pentose?

A

by N-B-glycosyl bond

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6
Q

How are each nucleotide connected?

A

phosphodiester bond to the 3’

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7
Q

5’ end

A

free phosphate group

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8
Q

3’ end

A

free hydroxyl group

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9
Q

Chargaff’s Rule

A

number of purines always equals the number of pyrimidines

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10
Q

Adenine bonds with

A

Thymine

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11
Q

Guanine bonds with

A

Cytosine

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12
Q

DNA is a ________ double helix

A

right handed

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13
Q

two DNA strands are…

A

antiparallel

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14
Q

1 turn of a helix includes…

A

10 pairs

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15
Q

palindrome

A

sequence that reads forward and backwards the same way

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16
Q

Palindromes can produce…

A

cruciform structures in DNA

17
Q

linear sequences of nucleotides in DNA encodes….

A

amino acid sequences of proteins

18
Q

TAA

A

stop

19
Q

supercoiling

A

intrinsic twisting of the double helix

-does not require DNA-protein interaction

20
Q

nucleosomes

A

consists of eight protein molecules called histones of which DNA wraps around

21
Q

double helix reversibly unwinds at temperature of…

A

60 degrees C

22
Q

melting temperature of DNA (tm) depends on…

A
  • temerature at which 50% is denatured
  • G+C content
  • different organisms
  • pH
  • salt conc.
23
Q

deamination of nitrogenous bases

A

results in wrong base pairings and can lead to mutations

24
Q

secondary structures of RNA

A

-hairpins, loops, bulge, double helix

25
Q

restriction endonucleases (restrictases)

A

produced by specific bacterial cells and cut DNA at specific short sequences

26
Q

DNA ligase

A

links DNA fragments together

27
Q

DNA polymerase

A

synthesizes complementary strands on a single strand DNA template

28
Q

-dedeoxy-nucleotides have no…

A

3’ hydroxyl which is needed to grow chain