Chapter 2 Flashcards
Graduated cylinders capacity range
From 5 mL to 5 L.
Larger graduated cylinders accuracy
Less accurate due to larger meniscus sensitivity to atmospheric pressure changes.
Volumetric flasks accuracy
Within 0.04% of the target volume.
Graduated cylinders accuracy
Within 1% of the target volume.
Common volumetric flasks capacity range
From 10 mL to 2 L.
Equipment for measuring 1 to 25 mL
Vacuum-assisted pipets.
Micropipets accuracy
Varies from 0.5-4% depending on size and volume.
10 mL disposable serological pipet accuracy
Measures to within 0.15 mL.
Pipette accuracy
Least accurate when measuring a volume at the bottom of its range.
Burettes typical volume range
Between 10-100 mL.
Burettes measurement range
Accurately measure volumes between 10-100 mL.
25 mL burette accuracy
±0.06 mL.
Erlenmeyer flasks measurement accuracy
Not used for precise measurements as their graduations provide only an approximate measure within 5% of the target volume.
Erlenmeyer flasks capacity range
From 25 mL to 4 L.
Common laboratory balances accuracy
Measure to the hundredth of a gram.
Typical temperature for biology experiments
37°C, normal human body temperature.
Room temperature in lab
Approximately 22°C.
Ultralow freezer temperature
-80°C or a liquid nitrogen tank at -196°C.
Storage temperature for temperature-sensitive reagents
4°C in a refrigerator or -20°C in a freezer.
Standard autoclave conditions
121°C and 15-20 PSI to kill bacteria and spores.
Autoclave purpose
Sterilizing liquids, containers, and instruments by increasing pressure to raise the boiling point of water beyond 100°C.
Organizations with BSL-2 laboratories
Biotechnology companies, research universities, hospitals, and national labs.
Agents in BSL-1 laboratories
Agents not typically associated with human disease
Cleanroom protective clothing
Hood, face mask, gloves, boots, and coveralls or a cleanroom gown.
