Chapter 3: Prokaryotic Cells Flashcards

(76 cards)

1
Q

Prokaryotes

A

unicelluarl; lack a membrane bound nucleus; lack membrane bound organelles

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2
Q

Monomorphic Bacteria

A

have one shape

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3
Q

Pleomorphic Bacteria

A

can take on different shapes/forms; this enhances their survival and appears to be important for transmission to a new human host

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4
Q

Prokaryote Sizes

A

rang from 0.2-750 micrometers in diameter; but average 0.5-2.0; mycoplasma species are some of the smallest; thiomargarita magnifica are some of the largest

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5
Q

Why are prokaryotes small?

A

surface area to volume ratio; they use diffision and a small size gives them a larger surface area voume ratio

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6
Q

Bacilli

A

singular: bacillus; rod shaped

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7
Q

Cocci

A

singular: coccus; spherical shape

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8
Q

Vibrio

A

comma shaped

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9
Q

Stella

A

star shaped

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10
Q

Coccobacilli

A

ovoid

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11
Q

Spirochetes

A

spiral shaped, corkscrew motion

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12
Q

Diplococci

A

paired cocci

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13
Q

Streptococci

A

chains of cocci

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14
Q

Staphylococci

A

grapelike clusters

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15
Q

Diplobacilli

A

paired bacili

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16
Q

Streptobacilli

A

chains of bacilli

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17
Q

Palisade

A

clusters of bacilli

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18
Q

Binary Fission

A

how prokaryotic cells reproduce; form of asexual reprduction which produces two “daughter” cloned cells

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19
Q

Steps of Binary Fission

A

DNA is copied; cell grows; copied chromosome is drawn to opposite ends of the cell’ septum (partition) begins to form at the midpoint;septum eventually walls off the resulting two daughter cells from one another

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20
Q

Extracellular structures

A

structures outside of the plasma membrane

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21
Q

Intracellular Structures

A

structures that lie within the boundary defined by the plasma membrane

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22
Q

Plasma Membrane

A

thin, flexible, phospholipid bilayer; selective barrier; proteins can constitue half of the plasma membrane mass; also a site for metabolic reactions (ETC) to make ATP

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23
Q

Selective Permeability

A

gases, water and small (nonchargged)substances can diffuse in and out of the cell; ions and larger polar substances require protein transporters to enter or exit a cell

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24
Q

Factors that Affect Fluidity of Plasma Membrane

A

temp (warm increase fluidity and cold reduce fluidity); fatty acid content (unsaturated fatty acid improve fluidity; saturated fatty acids are more rigid in cold)

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25
Bacterial Plasma Membrane
linear fatty acids
26
Archeal Plasma Membranes
long branched fatty acids; certain archaea that live in extreme heat build lipid monolayers as opposed to lipid bilayers
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Cell Wall of Bacteria
peptidoglycan as a core component of their cell walls; interchanging NAM and NAG that create a mesh like structure; LETS ANY thing thorugh NOT semipermeable
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Cell Wall of Archeae
Pseudopeptidoglycan is core component
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Gram Negative Bacteria Cell Wall
thin peptidoglycan layer; periplasmic space; outermembrane; contains lipopolysaccharid LPS; stain pink
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Gram Positive Bacteria Cell Wall
thick peptidoglycan layer; lack an outer membrane; stain purple
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Gram Negative Species in the Clinical Setting
typically harder to kill with chemical agents than gram positive; ourter membrane is a selective barrier tha guards against: damage to certain agents (lysozyme); variety of drugs; some detergents and disinfectants; contain porins
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Porin
protein channels that form a pore; nonspecific channels that allow substances to pass through the outer membrane; exclude large molecules and a variety of substances that may be harmful to the cell
33
Pros and Cons of Gram Positive Cell Walls
Cons: more sensitive to compounds that interfere with peptidoglycan; Pros: retain moisture longer; provide protection from mechanical stresses; presence of teichoic acids (stabilize wall, help maintain shape, transporit cations, and air regulation of division)
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COMPARISON TABLE FOR GRAM POSITIVE AND NEG CELL WALL
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Acid Fast Staining
acid fast staining detects a waxy lipid called mycolic acid in cell walls; appear red follwing acid fast procedure; ex) Genera Nocardia and Mycobascterium; nutrients and gases do not readily cross the waxycell wall therefore acid fast bacteria grow slowly;
36
Acid Fast Bacteria and Drugs
drugs do not easily corss the waxy cell wall therefore long and multidrug therapies in pateints infected with acid fast pathogens is necessary
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Mycoplasma Species
lack a cell wall; contain a sterol enriched plasma membrane; normally live inside other cells; pleomorphic
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L-Form Bacteria
had a cell wall and then lost it; may contribute to persistent infections since many antibiotics target cell wall structures (and they can kinda hide); resistant to certain environmental stresses (boiling autoclaving)
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Diffusion
the PASSIVE movement of substances from areas of high concentration to areas of concentration;
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Simple Diffusion
small noncharged molecules, gases and lipid soluble substances enter and exit cells
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Facilitated Diffusion
moves substacnces along their concentration gradient with the help of membrane proteins; channel proteins and carrier proteins;
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Osmosis
occurs as water is attracted to solute an dmoves from area of low solute to high solute; aka diffusion of water
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Hypertonic Solution
cause cells to lose water to their environment ; plasmolysis results whe the plasma membrane withdraws from the cell
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Hypotonic Solution
cause cells to gain water from the environment; osmotic lysis occurs if the bacteria cell wall is damaged or missing
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What are the three types of active transport prokaryotes use?
primary active transport; secondary active transport; and phosphotransferase systems
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Primary Active Transport
usues ATP to drive transport
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Secondary Active Transport
uses an ion gradient to drive transport; coupled transport or co-transport; symport, antiport
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Symport
ions and solute flow in the same direction
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Antiport
ions and solute flow in the opposite direction
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Phosphotransferase Systems
group translocation; high energy phosphate is transferred from a substrate onto the substance being transported; commonly used to import glucose in cell; eukaryotes only perform endocytosis
51
Flagella
use one or more for motility' filament like extracellular structure built from a protein called flagellin; works like a rotary propellor that spins from a rod and ring strucutre embedded in the cell wall; rotates 360 degrees not whip like
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Gram Positive Bacteria Flagella
possess two rings to secure the flagellum
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Gram Negative Bacteria Flagella
posses four rings used for anchoring
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Run and Tumble System
allows the cell to sense its environment and change direction; clockwise rotation of flagella results in tumble and counterclockwise results in a run; tumble used ot sense
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Chemotaxis
movement in response to a chemical stimulus
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Phototaxis
movement in response to light
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Aerotaxis
movement in response to oxygen levels
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Monotrichous
single flagellum
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Lophotrichouse
tuft or cluster of flagella at one poleA
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Amphitrichous
flagella at both poles of the cell
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Peritrichous
flagella all over the cell surface
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Periplasmic Flagella
located in the space between the plasma membrane and the cell wall; normally found in spirochetes to move in a corkscrew motion
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Fimbriae
short, bristle-like protein structure that extrude from the cell surface; adhesive properties help prokaryotes stick to surfaces or to each other for establishing biofilms; common in gram negative bacteria
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Pili
similar to fimbriae, except that they tend to be longer, more rigid, and less numerous; used to adhere to surfaces and aid in gene transfer via conjugation (sex pili)
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Glycocalyx
a sticky carbohydrate-enriched layer; helps bacteria stick to host tissues or surfaces, protects from desiccation; offers some protection against antibiotics and typical disinfection
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Slime Layer
unorganized and loosely associated glycocalyx
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Capsule
well-organized, tightly associated glycocalyx
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Prokaryotes still have intracellular structures such as
cytoplasm; most biochemical reactions occur in the cytoplasm; and inclusions used for storage
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Nucleoid
prokaryotic DNA is organized in a single, circular chromosome located in the nucleoid region of the cell
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Ribosomes
made of RNA and protein; build proteins by linking amino acids; prokaryotic 70S ribosomes are made of 2 subunits (large subunit 50S and small subunit 30S); dont add to 70 because the measurements are sediment not mass
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Cytoskeleton
composed of long protein filaments and provide structure and support
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Inclusion Bodies
distinct collections of substances inside prokaryotic cells; insoluble granuels; sometimes bound in a membrane; used to store useful substances; ex) carboxysomes (contain carbon fixing enzymes) and magnetosomes (accumulate magnetic iron)
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Endospores
metabolically inactive structures that allow certain cells to enter a dormant state; highly resistant to environmental stresses (starvation, heat, drying, freezing , radiation); when conditions become favorable, spores germinate back into vegetative cells (actively growing cells)
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Medically relevant species that make endospores in the genuses....
bacillus, clostridium, and clostridioides
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Sporulation and Steps
process of forming a spore; 1) copy DNA; 2) packaging DNA, ribosomes, and special enzymes into the spore coat; 3) surround the spore core with several heat and chemical resistant layers; 4) release
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