Chapter 9 Flashcards
(17 cards)
Which displays quaternary structure and which displays only tertiary
Hemoglobin quaternary
Myoglobin tertiary
What is heme
Oxygen binding component of hemoglobin and myoglobin
What is 2-3 bisphosphoglycerate
Bind in the Center of hemoglobin tetramer
What is bohr effect
The regulation of oxygen binding by hydrogen ions and carbon dioxide
Why do we need coorperative binding of oxygen by hemoglobin
In lungs high partial pressure and when moves to tissues low so causes release
What does carbon monoxide do
Moved saturation curve left causes right binding and R state
What happens to saturation curve when increase 2,3-BPG
Moves right
What happens to saturation curve when increase pH or decrease CO2
Move left
What is the Taut state
Low energy structure
Low affinity to bind ligand
What are the 2 second states in coorperative binding
Taut state with one ligand bound
Or
Relax state
What is the effect of more ligand on binding
Push to relax state and increase affinity to bind
What do hill coefficient
Measure of coorperativity
n
What does positive coorperativity mean? Negative?
Pos
Binding 1 ligand promotes other binding
n> 1
Neg
Binding 1 prevents other
n <1
What is max coorperativity
n=x
What is MWC model
Two state
Taut and relax
What is KNF model
Binding one subunit alters adjacent subunits
T state is stabalized by salt bridge between
His and Asp
