Chromatography Flashcards
(6 cards)
1
Q
Stationary phase
A
silica plate
2
Q
Mobile phase
A
solvent or gas that moves over stationary phase
3
Q
Rf
A
___distance by component____
. distance moved by solvent
4
Q
What has longer retention times
A
compounds with greater adsorption
5
Q
disadvantages
A
similar compounds have similar retention times
unknown compounds have no Rf comparisons
solvents don’t always separate all components
6
Q
Why are TLCs not be visible to the naked eye
A
amino acids are colourless so UV needs to be made visible
