collection, isolation, identification of bacteriologic samples

Question 1

this is used for the determination of the antibiogram profile of the pathogen which will serve as basis in the selection of antimicrobials for treatment

Answer 1

samples

Question 2

what sample must be submitted in cases like abortion

Answer 2

whole fetus or any gross lesions from the fetus, fetal abomasal content, affected placenta or cotyledons

Question 3

sample for suspected cases of leptospiral abortion

Answer 3

20ml midstream urine from the dam preserved in 1.5ml formalin

Question 4

collect these sample as soon as possible after mastitis is first noticed

Answer 4

first squirt of milk is discarded and to collect, sterile narrow-necked collection bottle must be held almosthorizontally

Question 5

samples in case of abscesses

Answer 5

3 ml of pus with scrapings from the wall of the abscess

Question 6

in cases of abscesses, this will yield great result

Answer 6

pus recently formed abscesses

Question 7

urine samples are collected for

Answer 7

urinalysis, bacterial microscopy, culture for viable bacterial count

Question 8

how to collect urine samples

Answer 8

cystocentesis, by catheter or mid stream urine

Question 9

in getting samples from skin lesions, intact pustules or vesicles are _____

Answer 9

disinfected with 70% alcohol, allowed to dry, and material is aspirated with sterile syringe

Question 10

if ringworm is suspected, samples from skin lesions should be

Answer 10

hair is plucked from the lesion and the edge of the lesion is scraped with blunt scalpel blade until blood oozes

Question 11

blood cultures is used if

Answer 11

bacteremia is suspected

Question 12

guidelines in blood culture

Answer 12

observe strict aseptic precautions
take more than 1 sample within a 24 hour period
and blood should be added aspetically

Question 13

for anaerobic culture, what samples shoud be taken if blackleg or malignant edema is suspected

Answer 13

a piece of rib stripped from periosteum

Question 14

in suspected enterotoxemia, samples is

Answer 14

20ml of ileal content

Question 15

transport media of streptococcus spp

Answer 15

swab on transport media

Question 16

transport media for taylorella equigenitalis

Answer 16

amies transport medium containing charcoal

Question 17

moxarella bovis transport medium

Answer 17

blood agar

Question 18

criteria for presumptive identification of bacterial pathogens

Answer 18

colonial morphology and color
presence or absence of hemolysis on blood agar
gram stain reaction
motility
growth on mcConkey agar
reaction on the oxidation-fermentation test
reaction in catalase and oxidase test

Question 19

• serotyping is based on the immunological identification of surface antigens on pathogens

Answer 19

immunologic techniques

Question 20

immunologic technique is particularly useful in the identification of

Answer 20

e coli and other members of enterobacteriaceae
listeria monocytigenes
pasteurella multocida
actinobacillus pleuropneumoniae

Question 21

Antibody demonstration in serum samples provide evidence of exposure. But it does not necessarily confirm an aetiologic role for that agent in the animal sample

Answer 21

serologic techniques

Question 22

can be used for detection and enumeration of pathogenic bacteria.

Answer 22

molecular techniques

Question 23

But it does not necessarily confirm an aetiologic role for that agent in the animal sample.

Answer 23

molecular techniques

Question 24

examples of molecular techniques

Answer 24

nucleic acid hybridization and pcr

Question 25

a standardized set of well characterized phages can be used for further identification of pathogens

Answer 25

phage typing

Question 26

Phage typing is frequently used in

Answer 26

Staphylococcus aureus, Salmonella Thyphimurium, and Salmonella Enteritidis isolates.