myco

Question 1

this group of mycobacterium is Slow grower species-require number of weeks to produce observable colonies

Answer 1

tuberculosis group

Question 2

species under the tuberculosis group of myco

Answer 2

m. tuberculosis, microti, africanum

Question 3

the moree rapidly growing species of myco which is -atypical,anonymous,or nontubercular mycobacteria

Answer 3

runyons group

Question 4

how many days will the runyon group produce colonies

Answer 4

3-21 days

Question 5

mycobacteria included in mycobacterium complex

Answer 5

m. avium and intracellulare

Question 6

the environmental and free living myco bacteria were placed in runyon in what year

Answer 6

1959

Question 7

categories of runyon group were based on

Answer 7

rate of growth, colony morphology, pigment production

Question 8

-producing pigmented (yellow colonies) only after exposure to light;slow growing;requires 7
days or more for visible colony

Answer 8

photochromogenic froup 1

Question 9

producing yellow or orange pigment in the absence of light, slow growin

Answer 9

2. scotochromogenic

Question 10

-producing no or slight pigment with exposure to light; slow growing

Answer 10

nonphotochromogenic

Question 11

grow rapidly with visible growth in less than 7 day

Answer 11

variable pigmentation

Question 12

-inhibit phagosome-lysosome fusion within macrophage

Answer 12

sulfolipids

Question 12

virulence factor of mycobacterium

Answer 12

mycosides
glycolipids
mycolic acid
sulfolipids

Question 13

enhanced survival of phagocytosed mycobacteria; act to
scavenge and detoxify the toxigenic oxygen radicals that are produced by the macrophage oxidative burst

Answer 13

glycolipids

Question 13

toxic, contribute to granulomatous response;

Answer 13

glycolipids

Question 14

control of cellular permeability

Answer 14

mycosides

Question 15

associated with cord factor that inactivates mitochondrial membranes within phagocyte and also inhibit
chemotaxis;

Answer 15

mycosides

Question 16

also associated with wax D and various proteins that induce delayed –type hypersensitivit

Answer 16

mycosides

Question 16

excreted in respiratory discharges, feces, milk, urine and semen

Answer 16

myco bovis

Question 17

pathogenesis of m. bovis

Answer 17

Infection via respiratory and intestinal tracts→local multiplication extracellular and intracellular due to resistance to
phagocytic killings→infected host cell and bacteria reach local lymph nodes→thoracic duct→general
dissemination→macrophage activation kills some bacteria→macrophage aggregation contribute to tubercle formation
which may be encompass by fibrous layer→ lymphatic spread is retarded but continuous extension occurs via erosion of
bronchi, blood vessels or viscera to new area

Question 18

specimen from live animal in myco

Answer 18

aspirates from cavities, lymph nodes, biopsies, tracheobronchial lavages and the
centrifuged deposit from 50 ml milk from suspected mastitis case

Question 19

specimens of myco from dead animals

Answer 19

fresh and fixed samples of lesions or a selection of lymph nodes from a tuberculin reactor
with no visible lesions

Question 19

can also be stained with fluorescent dye and viewed under UV microscop

Answer 19

myco

Question 20

zn pos, slender, often beaded roots

Answer 20

m. bovis

Question 20

Media for the mycobacterium

Answer 20

egg-based lowenstein-jensen and stonebrinks

Question 20

isolation m. bovis

Answer 20

Selective decontamination to reduce fast-growing mycobacteria Digestion or liquefaction of mucus Centrifugation of samples to concentrate number of bacteria before smear preparation and culture

Question 21

acts as prophylaxis in zoos and animal parks (myco)

Answer 21

isoniazid

Question 22

vaccine for prevention of infestation of m. bovis

Answer 22

bgc (bacilli-calmette guerin

Question 22

Treatment, Prevention and control: myco

Answer 22

isoniazid bgs early detection

Question 22

Tuberculin Tests commonly used:

Answer 22

tuberculin-purified protein derivative

Question 23

most widely used tuberculin test

Answer 23

intradermal

Question 24

inoculation sites of intradermal in tuberculin testing

Answer 24

caudal folds, vulvar lips, side of neck

Question 24

: intradermal inoculation of regular and avian tuberculin at two different sites in the neck. Read at 72 hours by measuring swelling

Answer 24

comparative cervical

Question 24

mostly used in primates; dilution of regular ‘bovine’ tuberculin is inoculated intradermally into the upper eyelid

Answer 24

opthalmis

Question 24

Pathogenesis and Pathogenicity Mycobacterium avium subsp. paratuberculosi

Answer 24

ingestion of feces, contaminated food and water ingested bacteria is taken up by macrophage to the intestinal lymph node immune mediated granulomatous reaction on the submucosa with accumulation of large numbers of macropgahe and lymphocytes slough off submucosa chronic enteritis and diarrhea emaciation and death

Question 25

m. avium specimen to be collected from live animals

Answer 25

a small pinch biopsy from rectum or rectal scraping, biopsy from the mesenteric lymph nodes, fecal samples

Question 26

m. avium specimen to be collected from dead animals

Answer 26

fresh and fixed samples of a section of the ileocecal valve, mesenteric lymph nodes

Question 26

in direct microscopy of m. avium, Smears of fecal and ileocecal valve mucosa stained with ZN reveals ___ which indicates intracellular growth

Answer 26

arge numbers of short, red staining rods in clumps,

Question 26

m avium intravenous johnin test positive reaction

Answer 26

a rise in temp to 1.5 C or more

Question 26

serology testing for avium

Answer 26

Complement Fixation test for screening, Immunodiffusion, ELISA

Question 26

avium subsp. paratuberculosis has been isolated from tissues of several human patients with

Answer 26

crohn's disease

Question 26

growth rate of m. tuberculosis and m. bovis

Answer 26

3-8 weeks

Question 27

growth rate of m. avium

Answer 27

2-6 weeks

Question 27

growth rate of m. avium subsp. paratuberculosis

Answer 27

ver slow upto 16 weeks

Question 27

optimal incubation of myco

Answer 27

37

Question 27

atmospheric requirement of myco

Answer 27

aerobic

Question 27

colonial features of m. tuberculosis

Answer 27

rough, buff, difficult to break apart

Question 28

colonial features of m. bovis

Answer 28

cream colored, raised with central rougness, break apart easily

Question 29

colonial features of m. avium

Answer 29

sticky off white, break off easily

Question 30

colonial features of m. avium subsp paratuberculosis

Answer 30

small, hemisherical, some pigmented

Question 31

essential growth supplemet of m. avium subsp paratuberculosis

Answer 31

mycobactin

Question 32

effect of added sodium pyruvate in myco

Answer 32

no effect except in bovis which is enhanced growth

Question 33

effect of added glycerol in myco

Answer 33

tuberculosis and avium- eugonic bovis- dysgonic

Question 34

Formation of single or multiple granulomas or nodules of the skin 1-3 cm in diameter

Answer 34

mycobacterium lepraemurium

Question 35

Painless and move freely. May ulcerate and discharge a slight, serosanguineus exudate. It is though that cats acquire infection via rat bites.

Answer 35

mycobacterium lepraemurium

Question 36

causes food poisoning to humans

Answer 36

bacillus cersus

Question 37

causes sporadic abortion in cattle and sheep

Answer 37

bacillus licheniformis

Question 39

chromosomally coded virulence factors of bacillus anthracis

Answer 39

1.Extracellular proteases 2.Siderophores 3.Anthrolysin O 4.Manganes Ion ATP binding cassette 5. Phospholipase C

Question 40

pathogenicity of anthrax

Answer 40

Uptake of the spores by the host from the environment→ germination within the host and multiplication of the vegetative form to large numbers→ bacteremia and toxemia→ death→ shedding of bacilli in the environment→sporulation.

Question 41

Diseased animals due to anthrax may suffer from:

Answer 41

>sudden, unexpected death >mild anorexia or reduced milk production in case of cattle >steep rise in temperature >exudation of tarry blood from body orifices, failure of blood to clot, incomplete rigor mortis, splenomegaly in cattle from orifices at death

Question 42

how to kill spores of b. anthracis

Answer 42

autoclaving 121C/15 minutes amd dry heat 1500/60min. (not by boiling for under 10min)

Question 43

what type of disinfectants is useful for anthrax

Answer 43

aldehydes, oxidizing and chlorinating disinfectants, beta propiolactone, ethylene oxide

Question 44

what specie is highly resistant to anthrax

Answer 44

birds followedf by pigs and carnivores, then horses and humas

Question 45

most susceptible to anthrax

Answer 45

cattle, sheep goat

Question 46

sample specimen for anthrax

Answer 46

blood from ear or tal veins, edematous fluid, peritoneal fluid

Question 47

specimen for culture of anthracid

Answer 47

blood or homogenous spleen

Question 48

edematous swelling that is hot and painful later becomes old and painless

Answer 48

malognat carbuncle

Question 49

respiratory infections smong workers working with hides, woll and hair contaminated with spores

Answer 49

woolsorter's disease

Question 50

bacilli is susceptible to

Answer 50

penicillin, streptomycin, tetracycline, fluoroquinolones, and erythromycin

Question 51

what specie of bacili is motile, resistant to penicillin, hemolytic,

Answer 51

cereus