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1
Q

these are simple tests that can identify the bacterium up to a generic level

A

gram reaction

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2
Q

widely used for the routine staining of bacteria in smears

A

gram stain

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3
Q

useful for demonstrating dermatophilus congolensis, rickettsiae and borrelia species which stain blue

A

giemsa stain

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4
Q

especially useful for recognizing campylobacter species, brachyspira species and fusobacterium species which stain red

A

dilute carbol fuchsin

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5
Q

used for the identification of bacillus anthraic in blood smears

A

polychrome methylene blue

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6
Q

hot concentrated carbol fuchsin which penetrates mycobacterial cell walls is retained after acid alcohol decoloriation

A

ziehl-neelsen stain

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7
Q

unlike ziehl neelsen stain, this metjod employs dilute carbol fuchsin with decolorization by acetic acid

A

modified zhiel neelsen

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8
Q

macConkey may differ in the composition of ___

A

bile salts or presence of crystal violetu

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9
Q

supports the growth of all members of the eneterobacteriaceae but selectively inhibitory to tother gram negative bacteria

A

macconkey

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10
Q

allows differentiation of lactose fermenters from non-lactose fermenters

A

macconkey

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11
Q

this test detects enzyme catalase that converts hydrogen peroxide to water and gaseous oxygen

A

catalase test

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12
Q

the positive reaction of this test is the effervescence of oxygen gas within few seconds

A

catalase test

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13
Q

Routinely used as an aid in distinguishing between
staphylococcus from streptococcus

A

catalase test

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14
Q

in catalase test, which is positive. staphy or strepto

A

staphy

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15
Q

2 methods of catalase test

A
  1. Loopful of bacteria on clean
    microscope slide + a drop of
    3% hydrogen peroxide
  2. A drop of 3% hydrogen peroxide is added to a colony on the plate and another drop on the area of the agar plate without bacterial growth
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16
Q

this test depends on the presence of cytochrome c oxidase in a
bacterial cell

A

oxidase test

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17
Q

these are oxidase negative

A

anaerobes

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18
Q

these bacterai can be used as a positive control organism

A

pseudomonas aurUGINOSA

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19
Q

the majority of bacteria are motile by means of

A

flagella

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20
Q

Motility can be temperature –dependent and some bacteria tend to be motile at

A

ambient temp but not at 37 degree celsius

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21
Q

these can be added in the media to aid the detection of motility

A

tetrazolium salt

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22
Q

a composite medium for the determination of hydrogen sulphide and indole production and motility

A

SIM medium

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23
Q

medium used mainly for enterobacteriaceae

A

SIM medium

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24
Q

this test is used to determine the oxidative or fermentative metabolism of a carbohydrate by
the bacterium

A

oxidation-fermentation test

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25
The medium is semi- solid and usually contains glucose and bromothyl blue as pH indicat
o-f test
26
* Bacteria that requires atmospheric oxygen for growth and metabolism are
oxidative
27
Bacteria that can metabolize glucose under aerobic or anaerobic conditions are
facultative anaerobes
28
facultative anaerobes in o-f test is
fermentative
29
o-f test results
if unreactive, both opena nd sealed tube is green (bordetella) oxidation- open tube is yellow, sealed tube is green (pseudomonas) in fermentation, open and sealed tube is both yellow(aeromonas)
30
in lana test, a swab is impregnated with ____
l-alanine-4-nitroanilide
31
what is the reslt if the swab in lana test turned yellow
the bacterium is gram negative
32
medium to indicate hemolysis
blood agar
33
what does it mean if a viscous gel is forming in a KOH test
it is a gram negative bacteria
34
the majority of a gram positive bacteria is susceptible to
vancomycin. gram negative are resistant
35
what are the primary biochemical tests for the identification of bacteria
gram staining growth or absence of growth on MacConkey presence or absence of hemolysis on blood agar catalase test oxidase test motility test SIM medium KOH test O-F test LANA test susceptibiluty to vancomycin
36
enzymatic attack on sugar with acid and gas production (Durham tubes
carbohydrate fermentation test
37
tested for the ability of the microorganism to use citrate as sole carbon source
citrate utilization test
38
chiefly used in the differentiation of Gram-positive rod
citrate utilization test
39
what bacteria can utilize citrate for growth
aerogenes group
40
lacks transport system that would permit the citrate to enter the cell to be use
e coli
41
ability of an organism to liquefy or hydrolyze gelatin
gelatin liquefaction
42
43
what does gelatin liqeufaction indicates
gelatin loses its gelling properties and remains a liquid even at low temperature
44
- a i d s i n t h e i d e n tifi c a ti o n o f o r g a n i s m s w i t h i n t h e Enterobacteriaceae
decarboxylase test
45
The specific decarboxylase enzyme produced by an organism results in the breakdown of the amino acid concerned and the fermentation of the corresponding amino acid
ornithine-putrescine lysine-cadaverine arginine-agmatine glutamic acid-aminobutyric acid
46
tryptophan split to indole
indole test
47
together with VP test, this is useful in differentiating between coli-aerogenes group of enteric bacter
methyl red test
48
-it is a confirmatory test for the butylene glycol type of fermentation of bacteria
voges-prokauer test
49
it detects the presence of acetoin or acetylmethylcarbinol, which is an intermediate in the production of butylene glycol
voges-prokauer test
50
discuss the nitrate reduction test
nitrate-nitrite-nitrogen gas
51
-test for the production of the enzyme urease by bacteri
urease test
52
-used for the identification of Proteus organism as well as the differentiation of other microorganisms
urease test
53
Urease breakdown urea to form
ammonia nad co2
54
-fermentation of sugars, hydrogen sulfide production, gas formation (hydrogen and carbon dioxide)
triple sugar iron
55
-useful for presumptive identification of Salmonella
tsi
56
in tsi, utilization of the ferrous substrate is indicated by
production of black color
57
the black color in tsi is
hydrogen sulfide
58
in tsi, if the medium becomes yellow, it means
acid production
59
alkaline color of the medium in tsi is due to
peptone degradation
60
in tsi, gas formation will show
cracks, bubbles, and indentation of the medium
61
a basic medium where non fastidious bacteria grows
nutrient agar
62
it is suitable for demonstrating colonial morphology and pigment production. also used for viable counting methods
nutrient agar
63
an enriched medium which supports the growth of most pathogenic bacteria and is used for their primary isolation
blood agar
64
allows the recognition of haemolysin production
blood agar
65
allows differentiation of lactose fermenters and non lactose fermenters
macconkey agar
66
selective enrichment media used for the isolation of salmonellae from samples containing other gram negative enteric organism
selenite broth, rappaport-vassiliadis broth
67
a blood agar based selective medium used for the inocculation and recognition of streptococci
edwards medium
68
heat treated blood agar which supplies special growth requirements for the isolation of heamophilus species and for the culture of taylorella equigenitalis
chocolate agar
69
used for the identifiction of enterobacteriadceae
IMViC testh
70
what is imvic test
indole methylene red voges-prokauer citrate utilization

bacteriology (8 decks)

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