Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

BLOODBANK/IMMUNOHEMATOLOGY > Component Preparation > Flashcards

Component Preparation Flashcards

1
Q

Time for whole blood collection (450-500 mL).

A

8 to 12 minutes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Time to reject blood unit if clotting occurs during collection.

A

12 minutes or above.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Temperature for blood bank refrigerator storage.

A

1-6°C (QC: 1.5 to 5.5°C).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Temperature for storage of platelet concentrate.

A

Room temperature (20-24°C) with continuous agitation.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Storage temperature for plasma-containing components.

A

Frozen at -20°C or colder.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Time for soft spin centrifugation.

A

3000/3200 rpm for 2-3 minutes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Time for hard spin centrifugation.

A

3500-3600 rpm for 5 minutes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Storage period for blood components with AS-1, AS-3, AS-5, AS-7 additives.

A

42 days.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Rejuvenation solution used for RBC regeneration in the US.

A

Rejuvesol.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Time to submit whole blood collected at 1-6°C.

A

Within 24 hours.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Time to submit whole blood collected at 20-24°C.

A

Within 6 hours.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Temperature for RBC components during transport.

A

Wet ice.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Transport requirement for platelet and WBC components.

A

Without ice.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Time for processing and centrifugation of whole blood for PRP and RBC components.

A

2 to 3 minutes (soft spin).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Time for platelet poor plasma centrifugation.

A

5 minutes (hard spin).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Needle size for blood collection.

A

16 gauge needle.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Blood storage requirement for red blood cells post-transfusion.

A

More than 75% RBC survival within 24 hours.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Allergy to iodine: alternative antiseptic.

A

Chlorhexidine gluconate solution.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Allergy to iodine and chlorhexidine: alternative antiseptic.

A

70% isopropyl alcohol.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Time for rejuvenation of RBCs with solution after outdating.

A

1-4 hours at 37°C.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Fresh Whole Blood volume for preparation.

A

450-500 cc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Platelet Rich Plasma volume for preparation.

A

200-250 cc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Platelet Poor Plasma volume for preparation.

A

200 cc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Platelet Concentrate volume for preparation.

A

50-70 cc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
PRBC volume for preparation.
200-250 cc
26
FFP volume for preparation.
200 cc
27
Cryosupernate volume for preparation.
180-200 cc
28
Cryoprecipitate volume for preparation.
15-20 cc
29
Storage condition for Platelet Concentrate.
Room temperature with agitation (3-5 days)
30
Storage condition for Fresh Frozen Plasma.
Freezer temperature
31
Components in Cryoprecipitate.
FVIIIc, FI (fibrinogen), FXIII, vWF, fibronectin
32
Storage condition for Cryoprecipitate.
Freezer temperature
33
Components in Cryosupernate.
Same as FFP but decreased in FVIIIc, FI, FXIII, vWF, fibronectin
34
Procedure for Cryoprecipitate preparation.
Slow cold thawing process of Fresh Frozen Plasma
35
Platelet agitation duration for storage.
3-5 days
36
Method for separating Platelet Rich Plasma.
Soft spin
37
Method for separating Platelet Poor Plasma and Platelet Concentrate.
Hard spin
38
Method of Leukocyte Removal.
Centrifugation, Washing procedures (using saline or glycerol), Mechanical separation using leukoreduction filters
39
First generation leukoreduction filters size.
170 um
40
Second generation leukoreduction filters size.
20-40 um
41
Third generation leukoreduction filters size; removes 99.9% of WBC.
3-log filter
42
Components able to pass through leukoreduction filters: standard filter of 170-200 um.
Normal platelets, normal RBCs
43
Components removed by (microaggregate) leukoreduction filters; 20-40um.
Fibrin clots, giant platelets, macrocytes, large RBCs, large WBC fragments, most white blood cells
44
Leukoreduction Definition.
Removal of white blood cells (WBCs) from blood or blood components prior to transfusion
45
Residual WBCs per each whole blood or red blood cells after leukoreduction.
<5 x 10^6 WBCs
46
Residual WBCs per each platelet derived from whole blood after leukoreduction.
<8.3 x 10^5 WBCs
47
Recovery of original component after leukoreduction.
>85% of original component must be recovered
48
Leukoreduction Categories - Pre-storage.
Performed shortly after collection
49
Leukoreduction Categories - Post-storage.
At the patient’s bedside
50
Leukoreduction Filters Usage.
Microaggregate filter or leukoreduction filter
51
Device designed to count WBCs at exceptionally low levels for leukoreduction quality control.
Nagoette Chamber
52
Instrument used in leukoreduction quality control to count WBCs at low levels.
Flow Cytometer
53
Purpose of irradiation in transfusion?
Prevents transfusion-associated GVHD by inactivating T cells; expires in 28 days.
54
Minimum gamma radiation dose?
25 Gy (central); 15 Gy (any part).
55
Radioactive sources for irradiation?
Cesium-137 or Cobalt-60.
56
Irradiation quality control method?
Radiographic film label darkening.
57
Purpose of washing blood components?
Removes extracellular solution to prevent FNHTR, TRALI, and allergic reactions.
58
Storage for washed RBCs?
1–°6C, up to 24 hours.
59
Storage for washed platelets?
20–24°C, transfuse within 4 hours.
60
Indications for washing?
Allergic reactions, antibody, and substance removal.
61
Purpose of pooling blood components
Allows multiple blood components to be transfused in a single event.
62
Pooling of multiple RBC units
Not typically performed; other product types may be pooled.
63
Reconstituted whole blood composition
Type O RBC + AB plasma; used for neonatal exchange transfusions.
64
Expiration time for pooled products
4 hours.
65
Maximum storage duration for frozen RBCs
10 years.
66
Cryoprotective agents in RBC freezing
Penetrating (e.g., dimethylsulfoxide) and nonpenetrating (e.g., hydroxyethyl starch) agents.
67
Initial freezing temperature for high glycerol RBCs
-80°C.
68
Freezer type for high glycerol RBCs
Mechanical freezer.
69
Maximum storage temperature for high glycerol RBCs
-65°C.
70
Shipping requirements for high glycerol RBCs
Dry ice with 40% glycerol.
71
Freezing temperature for low glycerol RBCs
-196°C in liquid nitrogen.
72
Storage temperature for low glycerol RBCs
-120°C in liquid nitrogen (20% glycerol).
73
Saline concentrations used in deglycerolization
First: 12% saline; Second: 1.6% saline; Third: 0.09% saline with 0.2% dextrose.
74
Adjustment for sickle cell trait donors during deglycerolization
1.6% saline step is omitted.
75
Method for glycerol removal in a closed system
Automated removal of glycerol.
76
Expiration of deglycerolized RBCs in AS-3 at 4°C (automated closed system)
14 days.
77
Advantages of RBC freezing
Long-term storage, maintenance of RBC viability and function, low residual leukocytes and platelets, removal of significant plasma proteins.
78
Disadvantages of RBC freezing
Time-consuming process, higher cost of equipment and materials, storage requirements (-65°C), higher cost of products.

BLOODBANK/IMMUNOHEMATOLOGY (22 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions