Conventional DSBR - Non-Homologous End Joining Flashcards
(8 cards)
1
Q
Repairs DSBs in mammalian animals caused by what?
A
Exogenous causes.
2
Q
Where in the cell does it function?
A
The entire cell.
3
Q
Why is it a relatively simple mechanism?
A
It require just a few kew proteins.
4
Q
What is the disadvantage of NHEJ?
A
It is error prone and leads to deletions.
5
Q
Step 1 - NHEJ
A
Ku70-Ku80c complex binds ends of break.
6
Q
Step 2 - NHEJ
A
DNA-Dependent Protein Kinases (DNA-PK) are recruited and their catalytic subdomain stabilises the ends, inhibits break end resection and increases accessibility for additional repair factors to the break site
7
Q
Step 3 - NHEJ
A
Artimis trims overhangs on 5’ and 3’ ends if present. This is where large deletions may occur.
8
Q
Step 4
A
XRCC4-LIG4 complex ligate the broken ends.
