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Culture Media Flashcards

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1
Q

What are the steps to culture media dispensing/distribution for plated media

A

Weigh the lyophilized powder
Dissolution
Autoclave
Dispense

Note: Almost same with the tube media. Difference: Autoclave & Dispense

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2
Q

What are the steps to culture media dispensing/distribution for tube media

A

Weigh the lyophilized agar
Dissolution
Dispense
Autoclave

Note: Almost same with the tube media. Difference: Autoclave & Dispense

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3
Q

Nutrient Agar & Trypticase Soy Broth are examples of what type of media

Classification of media according to fxn

A

Simple/Supportive/General Purpose

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4
Q

Give 2 examples of the most common enriched media used in the lab

A

BAP
CAP

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5
Q

LIM’s Broth, Tetrathionate, Selenite F, Alkaline Peptone water are examples of

Classification of media according to fxn

A

Enrichment media

Enriched media: Solid + additional supplements
Enrichment media: Liquid w/out additional supplements

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6
Q

Crystal violet, Gentian violet, Bile salt, Carbolfuchsin are inhibitors of what type of organisms

(Gram positive, Gram Negative)

A

Gram positive

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7
Q

Potassium Tellurite, Sodium Azide are inhibitors of what type of organisms

(Gram pos or Gram Neg)

A

Gram negative

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8
Q

What is used to inhibit swarming of certain organisms?

A

Alcohol, Chloral Hydrate, Trimethoprim lactate

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9
Q

Phenyl/Ethyl Alcohol, Columbia Agar, Colistin nalidixic agar are selective culture media for?

A

Gram Positive organisms

Inhibits gram negative organisms

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10
Q

This is a selective media for M. tuberculosis

A

Lowenstein Jensen

A protein enriched media

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11
Q

What is the inhibitor used for Lowenstein Jensen Agar when cultivating M. tuberculosis

A

Malachite green

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12
Q

Selective media for Corynebacterium diphtheriae

A

Loeffler’s medium

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13
Q

Inhibitor used in Loeffler’s medium when cultivating Corynebacterium diphtheriae

A

Potassium Tellurite

*Inhbitor for gram negative bacteria

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14
Q

Selective medium for N. gonorrhoea

A

Thayer Martin, Modified Thayer Martin, Martin Lewis, New York City Agar, GC-LECT

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15
Q

Antibiotics used for inhibition in Thayer Martin Agar & Modified Thayer Martin Agar

A

Vancomysin
Colistin
Nystatin

Modified Thayer Martin: VCN antibiotics + Trimethoprin Lactate (Inhibits swarming)

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16
Q

Antibiotics used for inhibition in Martin Lewis

A

Vancomysin
Colistin
Anisomysin (Antifungal)
Trimethoprim

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17
Q

Antibiotics used for inhibition in New York City Agar

A

Vancomysin
Colistin
Amphotericin B (Antifungal)
Nystatin?
Trimethoprim

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18
Q

Antibiotics used for inhibition in GC-LECT

A

Vancomysin
Colistin
Anisomycin
Trimethoprim
Lincomysin (Gram neg inhibition)

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19
Q

Selective medium for Staphylococcus

A

Mannitol Salt Agar

Inhibitor: 7.5% NaCl
pH Indicator: Phenol Red
CHO: Mannitol

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20
Q

Selective differential for Vibrio

A

Thiosulfate Bile Sucrose Agar

CHO: Sucrose
pH Indicator: Bromthymol Blue

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21
Q

K. pneumoniae is what type of lactose fermenter

(Rapid, Late, or Non)

A

Rapid Lactose Fermenter

*EKE (E. coli, Klebsiella, Enterobacter)

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22
Q

Erwinia is what type of Lactose Fermenter

(Rapid, Late, or Non)

A

Late Lactose Fermenter

*Yana Eats Half Sugar Cookies
Yersinia, Erwinia, Hafnia, Salmonella arizonae, Shigella sonnei, Serratia, Citrobacter

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23
Q

Serratia is what type of Lactose Fermenter

(Rapid, Late, or Non)

A

Late Lactose Fermenter

*Yana Eats Half Sugar Cookies
Yersinia, Erwinia, Hafnia, Salmonella arizonae, Shigella sonnei, Serratia, Citrobacter

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24
Q

All Salmonella are Non-Lactose fermenter except

A

Salmonella arizonae

*Yana Eats Half Sugar Cookies (Mnemonic for Late-Lactose Fermenter)
Yersinia, Erwinia, Hafnia, Salmonella arizonae, Shigella sonnei, Serratia, Citrobacter

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25
Kluyvera & Cedecea are what type of Lactose fermenter | (Rapid, Late, Non)
Non-Lactose Fermenter ## Footnote *Sugar Can Kill My PaPa Ed Salmonella, Shigella, Cedecea, Kluyvera, Morganella, Proteus, Providencia, Edwardsiella
26
What does Lactose fermenters appear as on Eosin Methylene Blue Agar
Pink/Purple Colonies
27
MacConkey Agar is an example of a selective medium for?
Gram Negative Enteric Bacilli ## Footnote *My X Eats His Shit MacConkey, XLD, Eosin Methylene Blue, HEA, Salmonella Shigella
28
This organism's colony has a greenish metallic sheen on EMB agar
E. coli
29
Klebsiella appears _______ on EMB agar
Pink mucoidal colonies ## Footnote *Klebsiella capsule Forming (Some Killers Have Pretty Nice Capsules) Rapid Lactos Fermenter (EKE)
30
Enterobacter colonies appear _________ on EMB
Pink colonies w/ black centers | Enterobacter are NOT H2S +
31
What are the inhibitors used in MacConkey Agar
Bile Salt & Crystal Violet ## Footnote Inhibits Gram positive: Bile Salt, Crystal Violet, Gentian Violet, Carbolfuchsin
32
What inhibitors are present in HEA agar
Bile Salt ## Footnote Inhibits Gram positive: Bile Salt, Crystal Violet, Gentian Violet, Carbolfuchsin
33
Sugars used in EMB
Levine: Lactose Hot Harris: Sucrose *LeLa loves eating w/ Hot Sauce
34
Sugars used in MacConkey Agar
Lactose
35
Sugars used in HEA
Lactose, Salicin, Sucrose *LSS sa Her
36
What is the H2S indicator used in Salmonella Shigella Agar
Ferric Ammonium Citrate ## Footnote All H2S Media uses FAC as indicator EXCEPT FOR TSI which uses Ferric ammonium sulfide
37
Salmonella colonies appear as _____ on Salmonella shigella agar
Yellow w/ black centers ## Footnote *Salmonella non-lactose fermenter (Sugar Can Kill My PaPa Ed) H2S + (SPACE) *Salmonella Shigella Agar MS Neutral (MacConkey, Salmonella Shigella Agar)
38
Salmonella arizonae appear as ____ on Salmonella Shigella Agar
Red w/ black centers ## Footnote *Salmonella Arizonae late lactose fermenter (Yana Eats Half Sugar Cookies) H2S + (SPACE)
39
Sugars used in XLD
Xylose, Lactose, Sucrose
40
Alkaline Peptone water is a special media used for
Vibrio cholerae
41
Monur's tellurite taurocholate gelatin agar is a special media for
Vibrio cholerae
42
Venkatraman Ramakrishnan is a special media for
Vibrio cholerae
43
Elner's medium is a special media for
Clostridium
44
Medium of Duncan & Strong is a special media for
Clostridium
45
Medium of Phillips is a special media for
Clostridium
46
Alkaline Egg medium is a special medium for
Clostridium
47
Fildes blood digest agar is a special media for
Haemophilus *haemoPHI = FIldes
48
Firm agar is a special media for
Proteus & C. tetani *Swarming organisms
49
Glycerol saline transport medium is a special media for
Salmonella
50
Hoyle's tellurite lysed blood agar is a special media for
Corynebacterium
51
Wilson & Blair's Brilliant green bismuth sulphite agar is a special media for
Salmonella & Shigella
52
Phenol-Red Egg yolk polymyxin agar is a special media for
Bacillus Cereus
53
Pike's media is a special media for
S. pneumoniae, H. influenzae
54
Polymyxi B-lysoxyme-EDTA Thallous Acetate is a special media for
B. anthracis
55
Proteose Peptone Yeast Extraction broth is a special media for
Anaerobes
56
Salt cooked Meat Broth is a special media for
S. aureus
57
Smith-Noguchi Medium is a special media for
Treponema
58
Carrot broth is a special media for
Group B Strep
59
Wilkins-Chalgren Agar is a special media used as an Antimicrobial susceptibility test for
Anaerobes
60
Kouga Agar is a special media for
Strongyloides stercoralis
61
Transgrow medium is an example of what type of media
Transport Media ## Footnote Together with Jembec, Stuart & Amie, Cary Blair, Venkatraman Ramakrishnan
62
Agar depth for Mueller Hinton Agar
4mm
63
pH for Mueller Hinton Agar
7.2 - 7.4
64
Used in the differentiation of non-fermentative gram-negative bacteria, particularly Pseudomonas aeruginosa
Acetamide Agar
65
Enrichment medium used for cultivation of vibrio species from feces
Alkaline Peptone Water
66
Differential Isolation and presumptive identification of Group D streptococci and enterococci
Bile Esculin Agar
67
Cultivation of non-fastidious microorganisms, used for the determination of hemolytic reactions
Blood Agar Plate
68
Isolation of Bordatella pertussis and Bordatella parapertussis
Bordet-Gengou Agar
69
Enrichment for Legionella spp. Supports growth of Francisella and Nocardia spp
Buffered Charcoal-Yeast Extract Agar (BCYE)
70
Used to differentiate Enterococci from other Streptococci
Bile Esculin Agar
71
Highly selective medium used for isolation of Salmonella Spp
Bismuth Sulfide Agar
72
Selective medium for Campylobacter spp
Campy-blood agar
73
Selective holding medium for recovery of Campylobacter spp
Campylobacter thioglycolate broth
74
Selective for Yersinia spp. maybe useful for isolation of Aeromonas spp
Cefsulodin-Irgasan-Novobiocin medium (CIN)
75
Selective isolaton of gram positive cocci in heavily contaminated sample (stool)
Columbia Colistin-Nalidixic Acid Agar (CNA)
76
For isolation of Corynebacterium diphtheriae
Cysteine tellurite blood agar
77
Used for isolation and differentiation of Staphylococci based on mannitol fermentation and gelatinase activity
Chapman stone medium
78
Cultivation of Francisella tularensis
Cystine Heart Agar
79
Detection of coliform bacteria in water
EC medium ## Footnote aka E. coli broth
80
Used for cultivating Streptococci & Lactobacilli, particularly in dairy procedures
Elliker Broth
81
Enrichment medium for Leptospira
Fletcher's medium
82
Differential selective medium for the isolation and differentiation of Salmonella and Shigella spp from other gram negative bacilli
Hektoen Enteric Agar
83
Selective and differential medium for Gardnerella vaginalis
Human Blood Tween Bilayer
84
Isolation and growth of Corynebacterium spp
Loeffler's medium
85
Isolation and differential of lactose fermenting and non-lactose fermenting gram negative bacilli
MacConkey Agar
86
For the isolation and differentiation of E. coli 0157:H7 in stool specimens
MacConkey Sorbitol Agar
87
Selective differentiation of Staphylococci
Mannitol Salt Agar
88
Recommended for isolation of Lactobacillus spp.
MRS (deMan, Rogosa, & Sharpe)
89
Recommended for antimicrobial disc diffusion susceptibility testing
Mueller Hinton Agar (MHA)
90
Cultivation of bacteria & enumeration of organisms
Nutrient Agar
91
Selective for Neisseria gonorrhoeae
New York City Agar
92
Qualitative procedures for the isolation of Mycobacteria
Petragnani Medium
93
Enrichment and selective medium for isolation of Bordatella pertussis
Regan Lowe
94
Selective for Salmonella some Shigella spp
Salmonella-Shigella spp
95
Selective for Campylobacter & Helicobacter spp
Schaedler agar
96
Enrichment of isolation of Salmonella spp
Selenite agar
97
Selective for Campylobacter spp
Skirrow agar
98
Selective for N. gonorrhoeae & N. meningitidis
Thayer-Martin Agar
99
Selective and differential for Vibrio spp
Thiosulfate Citrate Bile Salt Agar
100
Selective & enrichment for Streptococcus agalactiae in female genital specimens
Todd-Hewitt broth
101
Enrichment broth used for sub-culturing various bacteria from primary agar plates
Trypticase Soy Broth
102
Isolation and differentiation of Salmonella and Shigella spp. from other gram negative enteric bacilli
Xylose Lysine Deoxycholate Agar

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