CULTURE MEDIA Flashcards
(33 cards)
Commonly used base media
Agar
any SUBSTANCE that has the nutritional rqmts. for bacterial growth
culture media
only 1 organism is grown
pure culture
1 or 2 organisms are grown
mixed culture
made of pure culture in -20 / 70degC
Stock culture
Inverted position inside the ref
Prepared media
Stored in room temperature
Dry/Dehydrated media
PRAS
Pre-reduced Anaerobically Sterilized Media
Anaerobic Culture
PRAS
Gas Pak Jar
Candle Jar
Consistency of Liquid media
broth
Consistency of Semi-solid media
0.5-1% agar
Consistency of solid media
3-2% agar
How the medium is dispensed
- Plated
- Tubed
- Slant
- Butt
- Butt/Slant
enhances the propagation of bacteria
Enrichment Media
Enrichment Media for Salmonella and Proteus
Tetrathionate broth
Enrichment Media for Vibrio spp.
Alkaline Peptone Water
Medium that contains nutritive supplement ex. is blood
Enriched Media
Warming of Thioglycollate medium
warm in water bath to remove oxygen used
in anaerobes; pink at the top
distinguishes organisms growing together by their differences in cultural characteristics
Differential medium
Example of Differential medium
Mackonkey, SSA, EMB
promotes the growth of desirable organisms but at the same time inhibiting the growth of the other microorganism
Selective Media
Example of Selective Media
BSA, SSA, MAC, XLD, MSA, CTA, LJ,HEKTOEN
distinguishes organisms growing together by their differences in cultural characteristics
Selective & Differential
Example of Selective & Differential
EMB, MacConkey, TCBS
