culturing bacteria Flashcards
(24 cards)
def of differential media
selective for group bacteria and contains indicator
differential media (4)
MAC, TSI, Hektoen-Enteric agar, mannitol salt agar.
macconkey
lactose fermenters will cause colonies to be pink. non-lactose will be clear.
hektoen-enteric, what bacteria? picks up on what dz, what color?
G - enteric pathogens. good for salmonella. sugar fermenters turn salmon colored. non-sugar turn blue/green
triple sugar iron
yellow indicates fermentation
red indicated no fermentation
mannitol salt agar
selects for Staphylococci. isolate staph aureus
yellow- organism ferments mannitol
red- no staph
2 selective medias
selenite broth and tetrathionate broth
selective media contain what to allow bacteria to grow better
nutrients
basic nutrient media with extra nutrients (blood, serum, egg)
enriched media
what select bacteria for phenylethel alcohol agar and macconkey
PEA- gram +
MAC- gram - rods
partial, narrow band of green/slimy around colony
alpha
complete hemolysis- produce clear zone
beta
no change in media surrounding colony
gamma
double zone hemolysis.
delta
which two differentiate strept. species
alpha and beta
3 parts to the initial plating technique
protocol, contamination, application
what is more permeable to most common abx
gram +
3 types of zones of inhibition
sensitive- clear zone
intermediate- small clear zone
resistant- nothing. abx doesnt affect.
lowest concentration of the abx that inhibits growth
minimum inhibitory concentration
several types of culture media
agar plates, agar tubes, slant tubes, broth tubes.
types of media (2)
enriched media, basic nutrient
2 types of basic nutrient media
nutrient agar
trypticase soy agar
2 types of enriched media
chocolate
blood agar plate
2 solid selective media
PEA and MAC
