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Flashcards in Cytology Deck (28):
1

Name the different sampling techniques

Fine needle aspirate

Impression smear

Tape strip

Swab

2

What is a fine needle aspirate useful for analysing?

Masses

Tissues

Sarcomas

Spleen

Lymph nodes

3

What are the advantages to performing a fine needle aspirate?

Quick and cheap to perform

Often requires no sedation for superficial tissues

Can be done with/without ultrasound guidance

4

What kinds of fine needle aspirate methods are there?

With aspiration

Without aspiration

5

Why would you use different methods for a fine needle aspirate?

With aspiration is ideal for tissues with more structure to them e.g. soft tissue sarcomas

Without aspiration is better used for vascular or soft tissues e.g. spleen, lymph nodes

6

What are impression smears useful for?

Ulcerated masses

Skin pathologies

Samples from excised masses

7

What would you use a tape strip to sample?

Most often for skin cytology

Especially for infectious agents such as Malassezia pachydermatis

8

What is the recommended technique for performing a swab sample?

First moisten swab with saline

Swab area/lesion/tissue

Use gentle rolling motion to apply to microscope slide

9

What should be avoided when performing a swab sample?

Do not rub collected cells onto microscope slide as this is likely to rupture them

10

What could a swab sample be used for?

Skin cytology including ears

Nasal

Ocular/conjunctival

Vaginal

Fistulae

11

What could be potentially seen when viewing blood microscopically?

Anisocytosis

Polychromasia

Monocytes

Neutrophils

Lymphocytes

12

Which sampling method is most appropriate for obtaining small samples of cells from inside organs or masses?

Fine needle aspirate

13

When is a fine needle aspirate considered inappropriate?

In patients with a coagulation defect or from highly vascular masses

14

When is a fine needle biopsy most appropriate?

For highly exfoliative tissues or those that will not require aspiration

15

When is a fine needle aspirate biopsy most appropriate?

For tissues that don't exfoliate well e.g. muscle and spindle cell masses

16

Which technique might you use to sample ulcerated masses, burst pustules or for obtaining samples from the cut surface of a mass?

Impression technique

17

What is the recommendation for staining samples taken from a greasy area such as the ear?

It helps to gently heat-fix the slide by warming its underside with a hairdryer/radiator

18

How should samples be sent off to a laboratory?

Slides should be unstained, labelled with patient name, number and date and packaged into a slide carrier to prevent damage

19

Which samples should be packaged separately and why?

Cytological samples should be kept separate from histopathological samples to prevent the fixing formalin fumes from destroying the cytology

20

What tissue is this?

Q image thumb

Lymph node

21

What kind of tissue is this?

Q image thumb

Blood

22

What sort of tissue is stained in this slide?

Q image thumb

Liver

23

What sort of tissue has been fixed here?

Q image thumb

Salivary gland

24

What tissue has been stained here?

Q image thumb

Spleen

25

What does this image show?

Q image thumb

Urine crystals

26

What considerations would be made when studying a urine sample microscopically?

Presence of CaOx monohydrate crystals

                      epithelial cells

                      leucocytes

                      RBCs

27

What size needle is used for performing a fine needle/aspirate biopsy?

22G

28