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HISTOPATH > DECALCIFICATION > Flashcards

DECALCIFICATION Flashcards

1
Q

Removal of calcium

A

Decalcification

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2
Q

What is the volume for decalcification?

A

20x

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3
Q

Specimen for decalcification

A

Bone
Teeth
Tuberculous lung
Atherosclerotic blood vessel

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4
Q

ACID DECALCIFYING AGENTS

A

Nitric acid
10% Aqueous nitric acid
Formol-nitric acid
Perenyi’s fluid
Phloroglucin-nitric acid

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5
Q

Most common qnd fastest acid decalcifying agent

A

Nitric acid

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6
Q

Used for heavily mineralized cortical bone

A

10% aqueous nitric acid

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7
Q

Softener and decalcifying agent

A

Perenyi’s fluid

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8
Q

For small pieces of bone and teeth

A

Von ebner’s fluid

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9
Q

Autopsy materials, bone marrow, cartilage and tissues for research purposes

A

Formic acid

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10
Q

Excellent bone decalcifier for enzyme

A

edta

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11
Q

Removes calcium ions from formic acid-containing decalcifying solution

A

Ion exchange resin

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12
Q

Calcium migrates towards the negative pole

A

Electrophoresis

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13
Q

Factors influencing rate of decalcification

A

Concentration
Volume
Fluid access
Size and consistency
Agitation
Temperature

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14
Q

Tissue softeners

A

4% aqueous phenol
Molliflex
2% hydrogen chloride
1% hydrogen chloride in 70% alcohol

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15
Q

Test to measure the completeness of decalcification

A

Physical or mechanical
X-ray
Chemical Method (Calcium oxalate test)

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HISTOPATH (6 decks)

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