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HISTOPATH > DEHYDRATION-CLEARING-IMPREGNATION > Flashcards

DEHYDRATION-CLEARING-IMPREGNATION Flashcards

1
Q

Removal of intercellular and extracellular water from tissue

A

Dehydration

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2
Q

Routine dehydrating with

A

70% ethanol

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3
Q

Embryonic tissue starts with

A

30% ethanol

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4
Q

Volume of dehydrating

A

10x

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5
Q

Ratio of dehydrating agent and tissue

A

10:1

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6
Q

They are both dehydrating and clearing agent

A

Diethylene dioxide and Tetrahydrofuran

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7
Q

Plants and animals

A

Butanol

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8
Q

Urgent biopsies

A

Acetone

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9
Q

Miscible with alcohol , toluene, and xylene

A

Petanol

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10
Q

Blood and tissue films

A

Methanol

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11
Q

Best dehydratinf solutions

A

Ethanol

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12
Q

Removal of alcohol from tissue and replace with a substance that will dissolve the wax

A

Clearing

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13
Q

What should be the refraction index

A

1.518

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14
Q

Most rapid clearing agent

A

Xylene

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15
Q

Carcinogenic and can cause aplastic anemia

A

Benzene

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16
Q

Better at preserving tissues but more expensive

A

Toluene

17
Q

Turns milky if incomplete dehydration

A

Xylene

18
Q

Used as oil immersion

A

Cedarwood oil

19
Q

For tough tissue speimen, nervous tissue, lymph nodes and embryo

A

Chloroform

20
Q

For embryo, insects and very delicate specimen

A

Aniline oil

21
Q

Causes minimum tissue shrinkage

A

Clove oil

22
Q

Similar to chloroform but cheaper

A

Carbon tetrachloride

23
Q

Removal of clearing agent and replaced by a medium that wil completely fill all tissue cavities

A

Impregnation

24
Q

Methods of impregnation and embedding

A

Manual processing
Automatic processing
Vacuum embedding

25
Q

Fastest method of impregnation

A

Vacuum embedding

26
Q

Four changes of wax every 15 mins

A

Manual processing

27
Q

Four types of impregnating and embedding medium

A

Paraffin
Celloidin
Gelatin
Plastic

28
Q

Simplest and most common, best embedding medium

A

Paraffin wax Impregnation

29
Q

Reduces infiltration and facilitates thin sectioning

A

Dimethyl sulphoxide

30
Q

Contains rubber

A

Tissue mat

31
Q

Used for impregnation without clearing, melting point 46-48 °C

A

Ester wax

32
Q

More elastic and resilient, MP: 56-57°C

A

Paraplast

33
Q

MP: 57-58°C

A

Embeddol

34
Q

For the eyes

A

Bio/aid

35
Q

Used for bones teeth and brains

A

Wet celloidin method

HISTOPATH (6 decks)

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