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HISTOPATH > TRIMMING, CUTTING > Flashcards

TRIMMING, CUTTING Flashcards

1
Q

Example of disposable Embedding mold

A

Paper boats
Peel away
Plastic ice trays

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2
Q

L shape strip or brast or metal

A

Leuckhart’s embedding mold

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3
Q

Series of interlocking plates

A

Compound embedding mold

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4
Q

Tissue is arranged in precise position in the mold during embedding

A

Orientation

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5
Q

Three essentials parts of microtomy

A

Block holder
Knife carrier and knife
Pawl, ratchet feed wheel and adjustment screw

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6
Q

Also known as cambridge by Paldwell trefall and simplest

A

Rocking microtome

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7
Q

Other name for sliding microtome

A

Adams

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8
Q

Most commonly used microtome

A

Rotary microtome

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9
Q

What specific type of sliding microtome when the block holder is moving?

A

Base sledge

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10
Q

What specific type of sliding microtome when the knife is moving

A

Standard sliding

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11
Q

Thickness of tissue sections

A

Paraffin sections- 4-6 u
Celloidin sections- 10-15 u
Ultrathin sections- 0.5 u
Disposable blades- 2-4 u

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12
Q

Describe biconcave knife

A

Measures 120 micra
Both sides concave
For paraffin embedded tissue using rotary microtome

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13
Q

Describe plane wedge knife

A

100 micra
Both side straight
For frozen section- extremely hard and tough specimen in paraffin blocks using base-sledge microtome

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14
Q

Plane-concave knife

A

25 micra
One side is flat, other side is concave
If less concave- celloidin embedded tissue using sliding microtome
More concave- paraffin embedded tissue using rotary and rocking microtome

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15
Q

Sharp cutting edge that cuts 2-4 u thick sections

A

Disposable blades

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16
Q

Describe glass knives

A

Trimming and semi-thin sectioning

17
Q

Used to block resin block for electron microscopy

A

Diamond knives

18
Q

Angle formed between the cutting edge, normally 27 to 32 °C

A

Bevel angle

19
Q

Angle formed by the sides of the wedge knives normally 14-15 °

A

Wedge angle

20
Q

Angle formed between the cutting facet presenting to the block and the surface of the block, normally 0 to 15 °

A

Clearance angle

21
Q

Removal of gross nicks

A

Honing

22
Q

Purpose of honing

A

To remove irregularities from the knife

23
Q

Direction in honing

A

Heel to toe (edge first)

24
Q

Counterpart of honing that removes burr

A

Stropping

25
Q

Direction and purpose of stropping

A

Toe to heel (edge last)
Polish and sharpen the cutting edge

26
Q

Best honing stones

A

Belgium yellow

27
Q

Badly nick knives

A

Fine carborundum

28
Q

More polishing effect

A

Arkansas

29
Q

Common lubricant used for honing

A

Mineral oil
Clove oil
Xylene
Liquid paraffin
Soapy water

HISTOPATH (6 decks)

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