Dehydration

Question 1

The best dehydrating agent (fast acting; mixes with water and organic solvents; penetrate tissue easily)

Answer 1

Ethanol

Question 2

Used if good-grade absolute ethanol is not easily available

Answer 2

Isopropanol

Question 3

Toxic; for blood and tissue films and for smear preps

Answer 3

Methanol

Question 4

For plant and animal microtechniques

Answer 4

Butanol

Question 5

Cheap, rapid dehydrating agent; for utgent biopsies; volatile: flammable

Answer 5

Acetone

Question 6

Excellent dehydrating and clearing agent; miscible to water, melted paraffin, alcohol, and xylene; expensive; toxic

Answer 6

Dioxane/Diethylene dioxide

Question 7

Dehydrates rapidly; not harmful to tissue; toxic by inhalation, skin contact and ingestion

Answer 7

Cellosolve/Ethylene glycol monoethyl ether

Question 8

Tissues can be transferred directly after fixing and washing; dehydrate sections and smears following certain stains

Answer 8

Triethyl phosphate

Question 9

Dehydrates and clears tissues; miscible to water and paraffin; toxic; eye and skin irritant; cause conjunctival irritation (6 months exposure)

Answer 9

Tetrahydrofuran

Question 10

3 additives that are added to dehydrating agents in duly hard tissues

Answer 10

1) 4% phenol
2) Molliflex
3) Glycerol alcohol mixture