Dendritic cells

Question 2

Are DCs good at sampling?

Answer 2

Yes

Question 3

On mature DC ________ interacts with CD28 on T cells

Answer 3

B7.1 or B7.2

Question 4

What are the four ways that DCs capture and uptake antigens?

Answer 4

Micropinocytosis
Receptor mediated endocytosis
Caveolae dependent endocytosis
Phaogcytosis

Question 5

Steps of micropinocyotosis

Answer 5

Formation of large vesicles from plasma membrane
Ruffling of membrane
Uptake

Question 6

What receptors do DCs express?

Answer 6

C-type lectins
Fc receptor
Scavenger receptors
Receptors for HSP

Question 6

Is micropinocytosis uptake specfic or not?

Answer 6

Not

Question 7

C-type lectins: m_____ receptor and D________ recognised g_________ proteins

Answer 7

mannose receptors and DEC205 recognised glycosylated proteins

Question 8

Examples of scavenger receptor

Answer 8

CD38 binds apoptopic bodies

Question 9

What can DCs phagocytose?

Answer 9

Almost any bacteria, Gram +ve cocci and bacilli, and mycobacteria
Yeast cell, fungal hyphae
Apoptopic and necrotic bodies

Question 10

What triggers DC maturation

Answer 10

DAMPs/PAMPs

Question 11

Danger signals are mostly recognised by what receptor on DCs

Answer 11

TLRs

Question 12

TLR2 binds?

Answer 12

Gram positive components of cell wall

Question 13

TLR3 binds?

Answer 13

dsRNA

Question 14

TLR4 binds?

Answer 14

LPS

Question 15

TLR5 binds?

Answer 15

Flagellin

Question 16

What are some indirect singals?

Answer 16

TNF-alpha
IL-1beta
Prostaglandin- E2

Question 17

TLR activation increases what process and why

Answer 17

Micropinocytosis to capture more antigen

Question 18

Where do migrating DCs move to?

Answer 18

Secondary lymphoid tissue

Question 19

What receptor expression increases upon maturation of DC

Answer 19

CCR7

Question 20

Langerhams cells interact with keratinocyes via what

Answer 20

E-cadherin

Question 21

What molecule expression is reduced upon maturation: E-_______. What are the implication

Answer 21

E-cadherin - losing anchoring molecule allows for movement

Question 22

What are the two ligands of CCR7

Answer 22

CCL21 and CCL19

Question 23

What is CCL21 expressed bt

Answer 23

endothelial cells of lymphatic vessels

Question 24

What is CCL19 expressed by and where

Answer 24

Stromal cells in T cell zoen of lymph nodes

Question 25

Describe protease activity in iDC and mature DC

Answer 25

iDC have low protease activity and mature DC have high protease activity, effective at chopping up invariant chain and antigens.