Dengue

Question 1

Also known as

Answer 1

Breakbone fever

Question 2

A mosquito-borne infection caused by a
…

Answer 2

Flavivirus

Question 3

Transmitted by (2)mosquitoes.

Answer 3

Aedes aegypti and Aedes
albopictus

Question 4

• Characterized by….

Severe forms of the disease, (2) syndrome, principally affect children.

Answer 4

fever
severe headache
muscle and joint pain
nausea and vomiting
eye pain
rash

dengue hemorrhagic fever, and dengue shock

Question 5

• Dengue is endemic in more than____ countries, especially in the Philippines.

Answer 5

100

Question 6

Size and markings of Aedis aegypti

Answer 6

• Black, small to medium in size.
• Lyre-shaped silver markings on the mesonotum.

Question 7

Aedis aegypti leg marking

• Leg markings:

• Fore- and mid-legs:
• Hind legs:

Answer 7

Narrow white bands at the base of the tarsi.

Five broad white bands, leading to the name “tiger mosquito.”

Question 8

Aedis aegypti

• Breeding Sites (_____dengue):
• Prefers____ water.
• Found in indoor and outdoor containers:
Examples:

Answer 8

Urban

clear

Old tires, vases, jars, bottles.

Question 9

Aedes albopictus

• Identification:

Answer 9

Single, longitudinal silvery stripe on the mesonotum.

Question 10

Aedes albopictus

• Breeding Sites (_____dengue):
• Prefers_____ water.
• Found in indoor and outdoor
containers:
• Examples:_____

Answer 10

Rural

clear

Bamboo stumps, empty coconut shells, artificial containers, tree holes.

Question 11

Causative agent

Answer 11

Dengue virus

Question 12

VECTORS OF DENGUE IN THE PHILIPPINES

Answer 12

• Aedes albopictus

• Aedes aegypti

Question 13

DENGUE VIRUS
• A single-stranded RNA virus of the… family

Answer 13

Flaviviridae family

Question 14

T or F

• Widely present in tropical and subtropical
regions.

Answer 14

True

Question 15

T or F

• High morbidity and mortality, making it a major arthropod-borne viral disease.

Answer 15

T

Question 16

• Serotypes.
• Four main serotypes: (4)
• ______ was detected in____ (October 2013).

Answer 16

DENV-1, DENV-2, DENV-3, DENV-4

DENV-5 ; Malaysia

Question 17

Genomic Structure:
• Dengue virus genome translates into a single polyprotein encoding:
• 3 structural proteins:

Answer 17

Capsid (C)
pre-membrane (prM/M)
envelope (E)

Question 18

Genomic Structure:
• Dengue virus genome translates into a single polyprotein encoding:

• 7 nonstructural proteins:

Answer 18

NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5.

Question 19

• Found at high concentrations in sera of
infected patients during the early clinical phase.

Answer 19

NS1 ANTIGEN

Question 20

A highly-conserved glycoprotein secreted into the blood during dengue infection.

Answer 20

NS1 ANTIGEN

Question 21

• Detectable in primary or secondary infections from _____days after fever onset.

Answer 21

NS1: 1-9 days

Question 22

Positive NS1 test indicates what????

Answer 22

confirms dengue infection but does not identity the serotype

Question 23

IgM
• Primary dengue infection: Detectable
_______ after illness onset
• Secondary dengue infection: Detectable ______after illness onset

Answer 23

5-10 days

4-5 days

Question 24

is immunochromatographic assay designed to detect both dengue virus NS1 antigen and IgG/IgM antibodies against dengue virus in human whole blood, serum or plasma.

Answer 24

Bioline™ Dengue Duo

Question 25

Bioline™ Dengue Duo is ________designed to detect both dengue virus NS1 antigen and IgG/IgM antibodies against dengue virus in human whole blood, serum or plasma.

Answer 25

immunochromatographic assay

Question 26

Bioline dengue duo • Ideal diagnostic tool to cover all clinical stages from _____ to _____ • Presumptive differentiation between _______ and _____dengue infections

Answer 26

acute phase to convalescence phase primary & secondary

Question 27

Dengue duo • Easy to use rapid test (Test result:______) • Shelf life and storage temperature:_____ from the date of manufacturing at_____ temp

Answer 27

15-20 minutes 24 months 1-30 °C

Question 28

• Purpose: Detects dengue virus NS1 antigen qualitatively.

Answer 28

DENGUE DUO TEST NS1 ANTIGEN

Question 29

NS1 ANTIGEN • NS1 antigen in the specimen binds to_______ antibodies conjugated with______ • The antigen-antibody complex migrates via_______. • Captured by immobilized antibodies, forming a colored Test Line (T)

Answer 29

***mouse monoclonal anti-dengue antibodies*** conjugated with ***colloidal gold.*** chromatography

Question 30

NS1 antigen • A_____ ensures the test is functioning properly and should always appear.

Answer 30

Control Line ("C")

Question 31

NS1 • Result Interpretation: Positive: Negative:

Answer 31

Both "C" (Control Line) and "T" (Test Line) are visible. Only "C" Line appears.

Question 32

Purpose: Qualitative detection of IgG and IgM antibodies to dengue virus.

Answer 32

IgG/IgM

Question 33

IgG/IgM Purpose: Qualitative detection of IgG and IgM antibodies to dengue virus. • Detects antibodies for all______ using recombinant envelope proteins.

Answer 33

four dengue virus serotypes

Question 34

IgG/ IgM Principle: • Anti-dengue IgG/IgM in the specimen reacts with… • The antigen-antibody complex migrates through____.

Answer 34

recombinant envelope proteins conjugated with colloidal gold chromatography

Question 35

IgG/ IgM • Captured by immobilized anti-human IgG and/or anti-human IgM, forming: "G" Line: Indicates presence of… "M" Line: Indicates presence of… A _______ensures the test is performed correctly and should always appear

Answer 35

Dengue IgG antibodies Dengue IgM antibodies Control Line ("C")