Diagnostic molecular methods by Dr. Raymond (Roche) Flashcards Preview

Molecular > Diagnostic molecular methods by Dr. Raymond (Roche) > Flashcards

Flashcards in Diagnostic molecular methods by Dr. Raymond (Roche) Deck (12)
Loading flashcards...

List 3 advantages of molecular diagnostics over traditional methods.

1. extremely sensitive- low levels detected, previously uncultured agents identified, and small changes monitored.
2. highly accurate- based on identifying a pathogen by its DNA or RNA sequence, differentiate genotypic variants.
3. fast results for timely clinical decisions


medical devices that analyze human body fluids, such as blood or urine, to provide information for the diagnosis, prevention, or treatment of a disease.

In vitro diagnostics (IVD)


IVD stands for

In vitro diagnostic kit


RUOs stands for

Research use only kits


IUOs stands for

investigational use only


ASR stands for

Analyte specific reagents


Intended for use in diagnostic application for identification and quantification of an individual chemical substance or ligand in biological specimens.



When provided with the number of molecules generated and the number of PCR cycles performed, calculate how many molecules were in the original samples.

Example: 4 cycles = 96 so starting would be 6


Purifies nucleic acids from 1 to 32 samples in 90 minutes. And allows automated assembly of PCR reactions in 96 well plates, PCR tubes, light cycler capillaries and A rings for cobas amplicor analyzer.

MagNA pure LC instrument


This instrument is FDA cleared for use only with the Factor V leiden kit and the Factor II kit. User must validate any other use themselves.

The MagNA Pure LC


Name the most commonly inherited coagulation disorder that is associated with venous thrombosis.

DVT- deep vein thrombosis - Factor V leiden mutation


List five environmental risk factors that predispose a person to deep vein thrombosis (DVT)

Prolonged bed rest, hormone replacement therapy, inactivity, childbirth, pregnancy, long airplane flights, surgery (especially knee and hip), and oral contraceptives.