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Flashcards in DNA metabolism Deck (6)
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1

What is the central dogma to biology?

Flow of genes -> Proteins

2

What are the 3 kinds of DNA replication?

  1. Semiconservative
  2. Conservative
  3. Dispersive

3

Explain the Meselson + Stahl experiment

Growth of e.Coli in heavy isotope N15 only.

Strand of DNA -> CsCl centrifuge density gradient = low density

Same "heavy DNA", then allowed to replicate in N14

Creates hybrid DNA [N14/N15] -> medium density

Undergoes second replication and forms low density N14 and hybrid

Products: 1/2 N14 low density and 1/2  N15/N15intermediate density 

4

What did the Meselson and Stahl experiment prove?

Semiconservative replication

5

What would the products of the Meselson Stahl experiment be if conservative replication took place?

  • 1/4 High density [N15]
  • 3/4 Low density [N14]

6

What would the products of the Meselson Stahl experiment be if dispersive replication took place?

All intermediate density dna! hybrid of [N14/N15]