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mcb block 2 > DNA REPLICATION > Flashcards

DNA REPLICATION Flashcards

1
Q

Initiation of prokaryotic replication is done by?

A

Initiation protien at ORI site

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2
Q

What is the function of TOPOISOMERASE ?

A

Breaks DNA double strand during replication

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3
Q

Function of SSB protien ?

A

Prevents dna from annealing

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4
Q

An example of type 2 isomerase?

A

GYRASE

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5
Q

What is re replication block in Prokaryotic replication ?

A

HDA protien hydrolysis DNA ATP to ADP
HEMI-METHYLATION BECOMES COMPLETLY METHYLATED

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6
Q

What are the fragmetsof lagging strand called ?

A

Okasaki fragmets

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7
Q

Which enzyme join okasaki fragmets by cutting the primers ?

A

5’ - 3’ Exonuclease of Dna poly 1

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8
Q

What does DNA ligase do ?

A

Binds two okasaki fragmets

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9
Q

Direction of dna replication .?

A

5’-3’

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10
Q

Which enzyme proofread Prokaryotic dna replication ?

A

3’-5’ Exonuclease of Dna poly 3

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11
Q

Enzyme that removes primer in DNA replication ?

A

5’-3’ Exonuclease of Dna polymerase 1

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12
Q

Two drugs used to block Dna gyrase?

A

Quinolones (Oxacin) and Fluroquinolones

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13
Q

Sliding clamp in eukaryotic replication ?

A

PCNA

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14
Q

Which complex initaties dna replication in eukaryoties?

A

ORC COMPLEX

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15
Q

Which enzymes separates DNA in eukaryotic replication ?

A

MCM HELICASE

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16
Q

Which enzyme creates the first RNA strand in eukaryotic dna replication ?

A

Polymerase Alpha primase complex

17
Q

Primary synthesis of eukaryotic replication ?

A

Dna polymerase delta or epsilon

18
Q

During elongation in eukaryotic dna replication, which enzyme removes the rna primers ?

A

RNASE H (or) FEN-1

19
Q

Gap filling in eukaryotic dna replication ?

A

Dna polymerase delta

20
Q

What is TERT in eukaryotic dna replication ?

A

TELOMERASE REVERSE TRANSCRIPTASE

Uses it’s own rna as template and protien as enzyme to overcome the loss of genetic material

mcb block 2 (15 decks)

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