PCR Flashcards
1
Q
What is Dna microarray (ACGH) used to identify ?
A
SMALL CHANGES IN COPY NUMBER
RED AND GREEN ARE MIXED
YELLOW - NORMAL
GREEN OR RED- HIGH
2
Q
What is microsatelite analysis by PCR used ?
A
Change in VNTR
Looks larger in PCR
3
Q
What is allele specific pcr used for ?
A
To detect small mutation (deletion and insertion)
Both forward and reverse primer should pair, If not it is positive
4
Q
What is multiplex pcr used for ?
A
To scan deleted exons
5
Q
Next generation sequence in used for ?
A
Short and massive parallel strands
Cannot target single gene
6
Q
What is Q-RT PCR used for ?
A
To determine RNA amount by producing CDNA 1ST
AMOUNT OF RNA IS PROPORTIONAL TO THE PRODUCT
