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Flashcards in DNA Replication Deck (15):
0

What are you synethesizing in replication

Dna

1

What is the template made from

Dna
*youre making dna from dna

2

Parent dna vs daughter dna

Parent is what youre replicating

3

Semiconservative

One old strand and one new strand

4

Dna Helicase

-Unwinds DNA helix and separates strands forming the replication fork at the origin

5

Single stranded binding proteins

Bind and stabilize the single stranded DNA

6

Primase

-lays RNA primers on the leading and lagging strand
-has to be done for DNA pol III can work

7

Dna pol III

-Elongates new strands complementary to the leading strand(continuously) and the lagging strand(discontinuouslsy) in the 5'-3' direction
-the fragments on the lagging strand are called Okazaki fragments

8

DNA ligase

-Joins the Okazaki fragments together
-seals the backbone

9

Dna pol I

Replaces the rna primers with dna

10

Topoisomerase

Relieves tension

11

Telomerase

-replicates the telomeres(end of the strand)
-it extends the strand so that another primer can be layed down and elongated
---- there is a little extra genetic info but nothing is lost
-the telomeres get shorter and shorter over cell division until you eventually start to lose genetic information
-doesnt happen inprokaryotes bc there is no ends

12

Dna pol I
Dna pol II
Dna pol III
Prokaryotes

Pol I- replaces rna primers and dna repair.
Exonuclease activity:3'-5' and 5'-3' exonuclease.

PolIII- elongation
Exonuclease activity: 3'-5'

13

Origin of replication in prok vs euk

Only one spot in prok, many in euk

14

Bi-direction prok and euk

Both are