DNA replication and repair

Question 1

They confirmed semi-conservative DNA replication through 15N and 14N isotope study

Answer 1

Meselson and Stahl

Question 2

Synthesized continuously, and discontinuously

Answer 2

Leading and lagging strand

Question 3

Major replicative polymerase

Answer 3

DNA pol III

Question 4

Keeps DNA from closing, stabilizes single strand DNA

Answer 4

Single-strand DNA binding protein

Question 5

played an important role as an experimental system for dissecting the mechanism of DNA replication

Answer 5

T4 bacteriophage

Question 6

Eukaryotes replicate their genome in small portions called. It ensures DNA is only replicated once per cell cycle

Answer 6

replicons
control points

Question 7

correction of mistakes that escape the DNA polymerase proofreading activity

Answer 7

Mismatch repair (MMR)

Question 8

Historically atomic radiation. induced mutation breeding where radioactive sources are used to induce desirable mutations in crop plants

Answer 8

Gamma Gardens

Question 9

When DNA lesion is not repaired before DNA replication,

Answer 9

translesion synthesis

Question 10

Development experimental systems in understanding replication of eukaryotes:

Answer 10

1. Isolation of mutant yeast and animal cells unable to produce genes products required for replictions
2. Analysis of structure mechanism of action of homologous replica proteins from archaeal species
3. Development of virto systems using cell extracts or purified proteins