DNA Structure And Replication Flashcards

0
Q

Who demonstrated DNA acts as the genetic material using T2 bacteriophage?

A

Hershey and chase

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1
Q

Components of chromosomes?

A

Nuclei acids and proteins

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2
Q

The radioactive isotope 32P labeled which component do the T2 bacteriophage

A

DNA

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3
Q

Radioactive isotope 35s labeled which component of the t2 bacteria phage

A

Protein coat

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4
Q

DNA strands running anti parallel is when

A

When one strand runs 5’-3’ and one runs 3’-5’

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5
Q

Because of the 2nM width of the DNA double helix only a ______ base can pair with a _____base

A

Purine, pyrimidine

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6
Q

____NA is double stranded ___NA is single stranded

A

D, R

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7
Q

Difference in sugar of DNA and RNA?

A

DNA has 2 oxygen atoms in the sugar, RNA has 3 in the sugar

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8
Q

Purines?

A

Guanine, adenine

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9
Q

Pyrimidines?

A

Cytosine, thyamine, uracil.

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10
Q

In a nucleotide the nitrogenous base is attached to the sugars____carbon and the phosphate group is attached to the sugars ____carbon?

A

1’ 5’

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11
Q

Nucleic acids are assembled in the ___ to ___direction.

A

5’ 3’

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12
Q

Which enzyme joins short segments (Okazaki fragments) into a continuous strand?

A

Ligase

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13
Q

Product of DNA replication

A

2 new double helices each consisting one old and one new DNA strand

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14
Q

The 1st step in DNA replication is catalyzed by what enzyme?

A

Helicase

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15
Q

The action of helicase creates?

A

Replication forks and replication bubbles

16
Q

Why is the new DNA strand complementary to the 3’ 5’ strand assembled in short segments?

A

DNA polymerase can only be assembled in the 5’3’ direction

17
Q

The synthesis of a new DNA strand always begin with the synthesis of

A

A short RNA primer complementary to a preexisting DNA strand

18
Q

What is responsible to catalyzing the formation of an RNA primer

A

RNA Pol (primase)

19
Q

What catalyzes synthesis of new DNA strands?

A

DNA polymerase

20
Q

What stabilizes separated DNA strands during replication

A

Single-strand binding protein

21
Q

An old DNA strand is used as a ____ for the assembly of a new DNA strand?

A

Complimentary template.

22
Q

Weakest bonds in a DNA molecule are between?

A

Base to base

23
Q

What type of bonds hold together the sugar phosphate backbone of DNA

A

Covalent

24
Q

In DNA replication the leading strand_____

A

Is replicated into the replication fork in the 5’ 3’ direction

25
Q

The lagging strand is replicated..?

A

Out from the replication fork

26
Q

The energy required to catalyze nucleotide addition during DNA replication is derived from?

A

Nucleoside triphosphates

27
Q

Who’s proposed model of DNA is still used?

A

Watson and crick

28
Q

The enzyme that reduces the stress cause by opening the double helix is called

A

Gyrase

29
Q

After the formation of a replication bubble, what is he correct sequence of enzyme use for synthesis of the lagging DNA strand?

A

Helicase, primase, DNA polymerase, ligase