Drug and lipid analysis systems

Question 1

Homogeneous competitive binding immunoassays
Alcohol dehydrogenase-based
Colourimetric

Answer 1

Automated assays performed in the core lab with a quick TAT

Question 2

Most common automated assay

Answer 2

Homogeneous competitive protein binding immunoassays

Question 3

FPIA uses

Answer 3

plane polarized light

[drug] is inversely realted to the amt of polarization

Question 4

PETIA uses

Answer 4

antibody bound drug-linked particles that form insoluble aggregates that cause light to scatter
[drug] is inversely realted to the amt of particle aggregation

Question 5

EMIT uses

Answer 5

drug-linked enzyme becomes bound to antibody and inactivated

residual enzyme is proportional to the concentration of the drug in the patient sample

Question 6

NAD and alcohol dehydrogenase enzyme are added as reagents

NADH absorbance at 340 nm is measured and related to ethanol concentration in the specimen

Answer 6

Alcohol dehydrogenase-based automated test for alcohol

Question 7

False positives for Alcohol dehydrogenase-based automated test for alcohol result from

Answer 7

NADH being released in liver failure

Question 8

Cholesterol and triglyceride test reagents are used to quantitate concentrations by use of a timed enzymatic end-point method w/ color measurement at 520 or 560 nm

Answer 8

Automated lipid measurement methods

Question 9

HDL automated lipid measurement

Answer 9

detergent solubilizes only HDL, releases it for subsequent enzyme reactions, and blocks other lipoproteins from interfering

Question 10

LDL automated lipid measurement

Answer 10

not available, use Friedewald equation

Question 11

Stationary phase of GC

Answer 11

rubbery silicone polymer

Question 12

mobile phase of GC

Answer 12

carrier gas

Question 13

Separation of components is based on

Answer 13

Ability to vaporize

Polar functional groups

Question 14

Resolution of GC is

Answer 14

better than HPLC

Question 15

Disadvantages to FC

Answer 15

low throughput

labor intensive

Question 16

Most common detector for GC

Answer 16

NP detector, carbon is used for ethanol/toxic alcohols

Question 17

Stationary phase for HPLC

Answer 17

Minute porous silica particles coated with a hydrophobic fuzz

Question 18

mobile phase for HPLC

Answer 18

solvent mixture

Question 19

Advantage to HPLC

Answer 19

permits separation of substances which might be degraded by heat or which cannot be vaporized

Question 20

MS monitors/scans:

Answer 20

an entire molecular mass range in which drug molecules and their fragments can be expected to occur

Question 21

Identification on MS is from:

Answer 21

retention time and fragmentation pattern

Question 22

Legally accepted results:

Answer 22

GC/MS

Question 23

Hexapole collision cell is in which system

Answer 23

GC/MS/MS