E. Coli Flashcards

1
Q

Characteristics of E. Coli

A

Lactose positive
Oxidative negative
Motile Rods

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2
Q

Pathogenicity

A

Facultative

Obligate pathogenic depending on age of host

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3
Q

O =

A

O Anitigen 26

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4
Q

H =

A

Flagella 11

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5
Q

F =

A

Fimbriae 41

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6
Q

K =

A

Capsule 60

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7
Q

ETEC

A

Enterotoxigenic

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8
Q

EHEC

A

Enterohemorrhagic

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9
Q

VTEC/STEC

A

Verotoxigenic/Shiga like toxic

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10
Q

EPEC

A

Enteropathogenic

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11
Q

RPEC

A

Rabbit pathogenic

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12
Q

APEC

A

Avian pathogenic

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13
Q

NTEC

A

Neurotoxigenic (cattle only)

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14
Q

F4

A

Only in pigs
Mannose resistant
Genes expressed at body temp

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15
Q

F5

A

Pigs and Bovine
Mannose resistant
Temp dependent
Only in young animals

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16
Q

F6

A

Neonatal animals

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17
Q

F18

A

Seen in edema

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18
Q

F41

A

Pigs and Bovine

Mannose resistant

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19
Q

Labile Toxin (LT)

A

Heat labile
Decrease Na+ absorption
Increase Cl- and HCO3+ secretion
Withstand 30 min at 60C

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20
Q

STa

A

Activation of guanylate cyclase system
Decrease Na+
Increase Cl- and HCO3+

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21
Q

STb

A

Increase prostaglandin E2

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22
Q

EAST1

A

Similar to STa

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23
Q

Stable Toxin

A

STa
STb
EAST1
Withstand 15 min at 121C

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24
Q

ETEC in Pigs - Symptoms

A

Smelly diarrhea

Dehydration

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25
ETEC in Pigs - Target
Neonates - 2-4 wks
26
ETEC in Pigs - Virulence Factors - Neonate
F 4,5,6,41 | STa,b EAST1, LT
27
ETEC in Pigs - Virulence Factors - Neo-Weaning
F4 | LT, STa,b, EAST1
28
ETEC in Pigs - Epidemiology
Disruption of equilibrium between maternal immunity and infection pressure Sow - MMA Piglet - Low birthweight
29
ETEC in Pigs - Pathogenesis
Ascending from lower bowel --> oral uptake by host --> SI colonization Requires - adhesion, multiplication, enterotoxins, secondary septicemia
30
ETEC in Pigs - Diagnosis
Agglutination test | PCR
31
ETEC in Pigs - Treatment
Hydration | Antimicrobial
32
ETEC in Pigs - Prevention
Keep infection pressure low and maternal immunity high Proper Hygiene Vaccination Selection of receptor free piglets
33
VTEC in Pigs - Symptoms
EDEMA - Swollen eyelids, hoarse voice, CNS symptoms | Anorexia
34
VTEC in Pigs - Virulence
F 4,18 | St2e
35
VTEC in Pigs - Epidemiology
Sow to piglet | Weaning --> stress, changes in intestinal flora, maternal immunity
36
VTEC in Pigs - Pathogenesis
Excretion --> infection pressure increases --> contamination of other piglets
37
VTEC in Pigs - Toxin
Hyper acute - enterorrhagia Acute - permeability blood vessels edema Slower - intravascular coagulation Endotoxins - Shock and acute mortality
38
VTEC in Pigs - Diagnosis
Clinical signs Pathologic findings Bacteriology Virulence factors
39
VTEC in Pigs - Treatment
Reduce stress, Feed composition, Flora adaptation | Not yet affected - antimicrobial therapy
40
VTEC in Pigs - Prevention
Reduce stress Feed composition Adaptation of flora - probiotics, vaccination Receptor negative animals
41
EPEC in Pigs
Rare Diarrhea at weaning Diagnosis - Isolation + PCR
42
UTI in Pigs - Predisposing Factors
``` Hygiene Water intake Obstipation Age Individual ```
43
UTI in Pigs - Symptoms
Anorexia Hematuria Vaginal exudate General Malaise
44
UTI in Pigs - Diagnosis
Bacteriology
45
UTI in Pigs - Treatment
Culling | Antibiotics
46
MMA of PPDS in Pigs - Etiology
Heredity Hormonal Feed Infectious agents
47
MMA
Metritis Mastitis Agalactiae
48
PPDS
Post Partum Dysgalactiae Syndrome
49
ETEC in Bovine - Symptoms
Diarrhea | Dehydration
50
ETEC in Bovine - Target
Less than 3 days old
51
ETEC in Bovine - Virulence
F 5,41,17 CS31A | STa,b
52
ETEC in Bovine - Pathogenesis
Orap uptake --> XXX intestine --> enterotoxin production --> secondary septicemia Immunity/infection pressure imbalance
53
ETEC in Bovine - Diagnosis
ELISA | Culture + Virulence factor
54
ETEC in Bovine - Therapy
Separate sick animals Provide hydration Antibiotics NSAIDs
55
ETEC in Bovine - Prevention
Hygiene - separate calves from mothers | Antibiotics
56
EHEC and EPEC (eae) in Bovine - Target
1-8 week old
57
EHEC and EPEC in Bovine - Symptoms
Mucoid diarrhea +/- blood | Mainly in large intestine
58
EHEC in Bovine Zoonotic?
Yes
59
Septicemia in Bovine - Virulence
Serum resistance Iron uptake systems Capsule F17, CS31A, CNF1 and CNF2
60
Septicemia in Bovine - Pathogenesis
Bacteria PO --> lymph tissue of head, throat, intestine | Umbilical cord --> septicemia colostrum
61
Septicemia in Bovine - Hyperacute
Acute mortality | Shock
62
Septicemia in Bovine - Acute
General symptoms | Fast mortality
63
Septicemia in Bovine - Slow w/ organ localization
Poly arthritis Pneumonia Pleuritis Menigo - encephalitis
64
Septicemia in Bovine - Target
Neonates - lack of colostrum
65
Septicemia in Bovine - Therapy
NSAID Antibiotics Gamma globulins
66
E Coli Cats and Dogs - Enteric
Isolation in feces
67
E Coli Dogs - Cystitis
Fimbriae - Type 1, F12,134 Specific serotypes 50% alpha-hemolytic
68
E Coli Cats - Cystitis
Rare
69
E Coli Cats and Dogs - Pyometra
Same strain as cystitis in dogs
70
E. Coli in Poultry
Most important bacterial disease in broilers and layers
71
E. Coli in Poultry - Pathogen type
Facultative
72
E. Coli in Poultry - Serotypes
O1, O2, O78
73
E. Coli in Poultry - Clinical Signs
Neonatal - Colibacillosis Respiratory - colibacillosis and septicemia Peritonitis
74
E. Coli in Poultry - Pathogenesis Neonatal
Yolk rest infection --> sepsis --> death Yolk rest infection --> Polyserosistis/airsac/pericardia --> growth retardation Contamination of egg shell Neonatal contamination Manipulation of chicks
75
E. Coli in Poultry - Pathogenesis Respiratory Colibacillosis
Excretion in feces --> increase infection pressure --> Inhale E. Coli --> resp. disease
76
E. Coli in Poultry - Pathogenesis Peritonitis in Layers
Endogenic infection Start of lay Chronic form in older animals
77
E. Coli in Poultry - Diagnosis
Pathological lesions | Isolation of E. Coli from organs
78
E. Coli in Poultry - Treatment
Antibiotics - susceptibility test
79
E. Coli in Poultry - Prevention
Improve hygiene Decrease stress Temp. Control Vaccines
80
E. Coli O78, O2 Causes
Leads to Scabby hip/necrotic dermatitis in broilers | Prevention - Lower animal density
81
E. Coli in Rabbits - RPEC
EPEC (eae) Diagnosis - symptoms, isolation of subtyping Antibiotics
82
APEC Virulence Factors
Serum Resistance Capsule Iron Acquisition LPS - LipidA
83
ETEC in Pigs - Virulence Factors - Weaning
F 4,18 | STa,b EAST1
84
EHEC Bovine Neonate Virulence
EAE + VT1 or VT2