Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Mic. > E2.1 > Flashcards

E2.1 Flashcards

(43 cards)

Start Studying
1
Q

Methods for culturing microg.

A

5 basic tech.
Media
Microbial growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Name the 5 basic tech.

A

Inoculate
Incubate
Isolation
Inspection
Identification

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Which 2 of the 5 basic tech. can be switched

A

Inoculate and incubate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Inoculation

A

Produce a culture (sample/grow on media)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Types of inoculation

A

Culture media (nutritional material)
culture
-Verb=microbe growth
-nown=observing microbe growth
Colony=clone bact. cells grow on solid culture media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Innoculation vs. inoculum

A

Inoculation=The process of putting the bact. on plate
Inoculum=microbes/sample amount that is placed on the culture media to initiate growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Isolation

A

sep. bact. growth in a into an indiv. cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

3 ways to isolate bact.

A

streak plate
Dilution
Spread plate(think about what we did in master’s)
Both are isolation tech.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

needed for isolation (materials)

A

Culture media
Petri dish
Sep. tools

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Pure vs. Mixed culture

A

Pure=1 type of microbe
Mixed=multiple types of microbes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Agar do to bacteria?

A

dilutes it

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

MC type of isolation and how do you get colonies

A

Streak plate=look @ the 3rd/4th quad. of plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

look @ serial dilutions

A

1:10 dilution=1 ml of H20 and 9ml of broth
1:100 dilution=1ml of 1:10 and 9ml of broth
1:1000 dilution=1 ml of 1:100 and 9 ml. of broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Pour plate

A

Dilution is made in melted agar
Count colnies w/in the agar
Adv=viable cells
Disadv.=dilution errors
-colonies w/in agar cnt be tested and heat sensitive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Properties of media

A

Physical
Chemical
Functional

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Physical state of media

A

Liq. (sln)
Solid (agar)
Semi Solid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

liq. sln of media (physical media property)

A

Broths=H20 BASED
-nutrients and TSB(Trypticase-soy) or thioglycollate broth
*sugar, prot., ions,
* might see a tubulent bact. culture
Milks=provides extra nutrients
-ej=litmus milk
Infusion=solid component that is not dissolving quickly
-release nutrients slowly
-brain heat infusion(BHI) or cooked meat borth (MC)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Solid agar(Physical medium property)

Study These Flashcards
A

Agar(1-5%)
-polysacch. from seaweed(red algae)
*helps thicken media
-most microbes cnt degrade agar(unlike gelo)
-liq. @100C and solidfy @ 40C
* solid @ 35-37C
Types of agar
-deeps(no slant in tubes which decr. O2 and lifespan)
-plate
-slats

19
Q

semi solid(Physical medium property)

Study These Flashcards
A

decre. conc. of agar (0.3-0.5%)
-clot like/gelatin agar and motility agar
Not liq. but not agar
Help define mobile bacteria

20
Q

Chemical medium property

Study These Flashcards
A

Synthetic or nonsynthetic
Nutrients=C, N, S, P
-peptone, proteins, AA, GF
Mosture
Adjust pH buffers
correct O2 level
Sterile

21
Q

Synthetic(chemical medium property)

Study These Flashcards
A

Grow small grp/related organism
-very specific microbe
Exact chem. composition (organic or not compounds)
-used to grow a specific microbe
Must contain organic GF for Carbon/E source
Not good for fastidious organism (ej=Neisseria spp.)

22
Q

Nonsynthetic chem. medium AKA

Study These Flashcards
A

COMPLEX MEDIUM

23
Q

Nonsynthetic (chem. medium)

Study These Flashcards
A

Not chem. defien/pure
Ej=help gives out nutrients
-Animal Extract
*Blood (sheepMC)
*Chocolate agar(Lysis RBC to release nutrients)
-plant extract=tryp soy agar(soybean extract)
-yeast extract
-Maconckey agar
Heterotrophic bact./fungi
E, C, N and S are supplied by protein

24
Q

Functional (medium properties)

Study These Flashcards
A

Gen. purpose
-good for complex medium and grow broad spectrum
-nutrient agar and browth
*brain-heart infusion and trpticase soy agar
types
-Enriched media
-Selective media
-Differential Media

25
Enriched(functional medium properties)
Complex(contain, blood, serum, GF ETC.) Good for fastidious organism (very picky/specific req.) Keep bact. growth low Ej=blood agar, and chocolate agar (complex)
26
Selective media (functional medium properties)
Only alows 1 type of microbe growth while supressing other Selective agent -pH -ion/dyes -antiBT Ej -mannitol salt agar(7.5% of salt) *most have 1% which helps staph A(halophiles) to grow -MacConkey/EMB=only gram - grows while inhibiting gram +
27
Define halophile
grow in high salt content
28
Differential Media(functional medium Properties)
See different types of Bact. -different bact. will change media
29
Blood Agar (differential types of media)
Blood Agar=detect hemolysis of RBC(all complex/enriched and diff.) -alpha=partial hemo. *Green halo around bact. colonies *e.Coli -Beta=complete hemolysis *clear halo around colonies *ej=strep throat/strep pyro -gamma=no hemolysis *no RBC brkdwn *ej=staph epi
30
MacConkey agar(diff. type of media)
Lactose fermenters(pink) -non lactose fermentiers=clear -has special dyes that inhibit gram + but allow gram -
31
Eosin methylen Blue agar (EMB)-diff. type of media
Lactose fermentation (~ to MacConkey) -selective for only gram -diff. -ej=E. coli w/ green metallic sheen and pink colonies
32
Mannitol Salt Agar
Monnitol fermenters(yellow/change pH) -Non mannitol fermenters=STAYS red -selective but also differential -ej=Staph. epi is red while Staph aureus is yellow
33
Other types of media
Anaerobic growth Carbo. Fermentation Transport media
34
Types of differential agar
Mannitol Salt MacConkey EMB Blood
35
Anaerobic growth (other types of media)
Reducing media=chemically combine/remove O2 -thioglycollate broth Allows anaerobic bact. to grow
36
Carbo. fermentation (other type of media)
Contain sugar/carbo and pH indicator Sugars are fermented to acid =pH indicator changes color
37
Transport media (other type of media)
Maintain and pre3serve specimens that have to be held for a period of time before clinical analysis Stuart's /amies transport media
38
Special type of Culture tech.
Growth of live animal=Mycobacterium leprae(armadillos) Treponema pallidum=syphillis spirochete -cnt grow on artifical media Rickettsia /chlamydia=intracell. bact. -MUST be grown in live cell culture Virusis=grown in live cell cutlure
39
Incubation
Time needed to grow media Also temp/atm control Capnophiles=require high conc. of CO2
40
Inspection
Pure culture Sub Culture Mixed Culture Contaminated Culture
41
Identification
Biochem. test. Colony appearance Immuno. testing etc.
42
agar does what to the bacteria in the petri dish
dilutes that bacteria to stop lawn
43
what type of loop is needed for the isolating the bacteria on the petri dish
innoculating loop

Mic. (4 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions