Enzymbiology

Question 1

What are enzymes,, What are some enzymes that are also RNA? What are some co-factors .

Answer 1

Question 2

What are the 6 types of enzymes ? Just names

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Question 3

What is the role of oxidoredutases and Transferases ?

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Question 4

What is the role Hydrolase and lyases ?

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Question 5

What is the role of isomerase ? and ligases ?

Answer 5

Question 6

What is the difference between transititon state complex and enzyme-substrate complex ?

Answer 6

Question 7

What are the substrate binding models ?

Answer 7

Question 8

What does an enzyme do to the activation barrier? How about free energy of reactions and products ? Does it change equilibrium ?

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Question 9

What do enzymes organize reactive groups ? What happens to uncatalysed bimolecular reactions ? Uncatalyzed unimolecular reactions ? How about catalysed reactions ?

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Question 10

What is the difference of the free energy of activation between catalyist and non catalyst ?

Answer 10

Question 11

Draw the energy changes that occur during a reaction between a substrate and enzyme .

Answer 11

Question 12

Why is there a dip ?

Answer 12

Bonds are beign formed, which is exothermic, that the energy released is used to decrease the energy of activation

Question 13

What is the transition state?

Answer 13

When half of the reactant got converted

Question 14

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Question 15

What are transition state inhibitors? What are transition state analogs ? What binds more tightly between the anaylog and the real subtrate ?

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Question 16

What is the role of pencicillin as a transition state inhibitors ? What does it interact with ?

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Question 17

Explain how allopurinol is an example of a transition state inhibitor ?

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Question 18

In the mechelis menton grapth, enzymes show what type of curve? While alloteric enzymes show what ??

What is the formula for V?

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Question 19

Where is Vmax, and KM ? What is the formula ?

Answer 19

Question 20

What is known as michelis complex ?

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Question 21

Vo=

When the substrate is higher then the km, is in what power ? What does it mean with VMAX ? How about when substrate is less ?

Answer 21

intial rate

Question 22

What does it mena when the Km is greater than another km in terms of affinity ?

Answer 22

Question 23

In a lineweaver burk equation what is it ? How are the x axis and y axis represented ? What is the slope ?

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Question 24

What is the formula for a single substrate ? v=

What is Kcat ? What is Km ?

Answer 24

Question 25

What are enzymes inhibitors ? What are irreversible inhibitors when are they used ?, and reversibile inhibitors ? What is the role of reversible inhibitors ?

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Question 26

name all the reversible inhibitors

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Question 27

What changes in a competitive inhibior between the km and Vmax ?

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Question 28

What is a non-competiive inhibitor, where does it bind ?

Answer 28

Question 29

What changes between km and Vmax in a non-competitive inhibitor ?

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Question 30

What changes in a non-competiitve inhibitor

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Question 31

Draw the mechanism by which uncompetitive inhibition occurs .

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Question 32

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Question 33

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Question 34

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Question 35

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Question 36

What is allosteric regulation ? What type of ligand ? How is it regulated ?What type of modification is it ? is it reversible ? What are the modifications ? What is proteolysis role ?

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Question 37

What is ment by allosteric enzymes having copertivity ?

Answer 37

Question 39

What is a positive allosteric enzymes and negative ? Why not confuse with line weaver

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Question 40

How does the VM and km compare in allosteric enzymes that are increase or decrease ?

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Question 41

Phosphorylation is a type of ? What does it do ? Can it be reversed ?

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Question 42

How do tissue regulate how much a certain protein they are making ? What is enzyme synthesis ? What is protein degradation ?

Answer 42