Enzymes Flashcards
How do enzymes catalyse reactions
Work by decreasing the activation energy of spontaneous reactions
They are substrate specific and are not used up in the reaction
Bin to the active site of the substrate then change configuration, help produce the product but aren’t involved
What can effect enzymes
PH, temperature, concentration, inhibitors
What is michaelis constant
A measurement of the affinity of the enzyme and affinity for one another
Explain Kmax and Vmax
Kmax is the affinity a substrate and enzyme have
Vmax is how fast a reaction is proceeding when substrate is fully saturated with enzyme
Explain competitive and non- competitive inhibitors
Competitive- Vmax unchanged, Kmax increased to overcome inhibition
Non- competitive- Vmax decreased, Kmax unchanged
