Enzymes

Question 1

What is the chemical nature of an enzyme

Question 2

What biomolecule are enzymes made from

Question 3

Explain how an enzyme is a biological catalyst

Question 4

State the shape of an enzyme

Question 5

List two factors that affect the activity of enzyme

Question 6

Describe what is meant by optimum activity in relation to enzymes

Question 7

Define the terms substrate and product

Question 8

To describe a catabolic enzyme and name an example

Question 9

To describe an anabolic enzyme and name an example

Question 10

To draw a graph showing how temperature affects enzyme activity

Question 11

To draw a graph showing how pH affects enzyme activity

Question 12

Define the term denatured enzyme

Question 13

Define the term immobilised enzyme

Question 14

Define the term bioprocessing

Question 15

Define the term bioreactor

Question 16

Name two products made from bioprocessing using enzymes

Question 17

To describe two methods of immobilising enzymes

Question 18

Name a gel used to trap an enzyme

Question 19

List three advantages of immobilised enzymes

Question 20

Name one example of an immobilised enzyme

Answer 20

the substrate it reacts with

Question 21

Name the enzyme

Answer 21

substrate

Question 22

State why only one factor should only change in an experiment

Question 23

State how you might change temperature

Question 24

State how you might keep the temperature constant

Question 25

State how you might change the pH

Question 26

State how you might keep the pH constant

Question 27

State why you used washing up liquid

Question 28

Name the source of catalase

Question 29

To describe how you measured the activity of enzyme

Question 30

State the control used in each experiment

Question 31

State how you might denature enzyme in the heat denaturation experiment

Question 32

To prepare an enzyme immobilisation state why you used yeast

Question 33

To prepare an enzyme immobilisation state why you used sodium alginate

Question 34

To prepare an enzyme immobilisation

Answer 34

state why you used calcium chloride

Question 35

To prepare an enzyme immobilisation state why you used the syringe

Question 36

To prepare an enzyme immobilisation

Answer 36

state why you stir the beads

Question 38

To prepare an enzyme immobilisation state why you rinsed the beads with distilled water

Question 39

To examine the application of immobilised enzymes state why you added sucrose to immobilised sucrase in separating funnel

Question 40

To examine the application of immobilised enzymes state the enzyme

Answer 40

substrate and product

Question 41

To examine the application of immobilised enzymes state how you tested for the product

Question 42

To examine the application of immobilised enzymes state the control

Question 43

To examine the application of immobilised enzymes state if the immobilised Sucrase or free sucrase formed the product fastest

Question 44

To examine the application of immobilised enzymes state why the immobilised sucrase formed a pure clear product

Question 45

To examine the application of immobilised enzymes state why the free sucrase formed a cloudy product

Question 46

Define the active site

Question 47

What is meant by enzyme specificity

Question 48

To explain how an enzyme works (induced fit model or active site theory)