Enzymes Reclec

Question 1

Act as biological catalyst

Answer 1

Enzymes

Question 2

Increases the rate of reaction

Answer 2

Catalyst

Question 3

Other term of inorganic cofactors of enzymes

Answer 3

Activators

Question 4

Two cofactors of enzymes

Answer 4

Inorganic and organic

Question 5

Other term for organic cofactors

Answer 5

Coenzymes

Question 6

Enzymes that does not have a cofactor

Answer 6

Apoenzymes

Question 7

Complete and active enzyme

Answer 7

Holoenzymes

Question 8

Enzymes’ inactive form

Answer 8

Proenzyme / zymogen

Question 9

Variants of enzymes

Answer 9

Isoenzymes

Question 10

Where does the substrate binds to?

Answer 10

Active site

Question 11

Where does the regulatory molecules binds?

Answer 11

Allosteric site

Question 12

Who developed or established the classification system of enzymes

Answer 12

Enzymes commission (international union of biochemistry)

Question 13

Enumerate the 6 classes of enzymes

Answer 13

1 oxidoreductases
2 tranferases
3 hydrolyses
4 lyases
5 isomerases
6 ligases

Question 14

Joining of two molecules that needs ATP

Answer 14

Ligases

Question 15

Redox reactions

Answer 15

Oxidoreductases

Question 16

Transfer atom/groups

Answer 16

Tranferases

Question 17

Removal of groups and creates double bond

Answer 17

Lyases

Question 18

The binding of an enzymes to a substrate is a ______

Answer 18

Physical absorption

Question 19

The substrate fits like a lock and the substrate binds exactly to the enzymes.

Answer 19

Lock & key theory

Question 20

It imply that your enzymes are rigid

Answer 20

Lock & key theory

Question 21

The active site of this theory undergo conformational change

Answer 21

Induced fit theory

Question 22

The widely accepted theory

Answer 22

Induced fit theory

Question 23

Dependent on substrate concentration

Answer 23

First - order kinetics

Question 24

Dependent in enzyme concentration

Answer 24

Zero-order kinetics

Question 25

One substrate will only bind to one enzymes

Answer 25

Absolute specificity

Question 26

The enzyme will only accept substrate that has a hydrogen bond

Answer 26

Bond specificity

Question 27

The enzyme will only accept the substrate that has the same group

Answer 27

Group specificity

Question 28

The enzyme can only accept substrate that has the same configuration

Answer 28

Steroeoisometric specificity

Question 29

6 factors affecting enzymes activity

Answer 29

1 enzyme /substrate concentration 2 pH 4 temperature 4 cofactors 5 inhibitors 6 interference

Question 30

All enzymes are bound to substrate

Answer 30

Saturation kinetics

Question 31

Optimal pH for most enzymes

Answer 31

7-8

Question 32

Alkaline phosphatase requires a pH of _____

Answer 32

9-10

Question 33

Acid phosphatase requires a pH of ___

Answer 33

5

Question 34

Acid phosphatase requires a pH of ___

Answer 34

5

Question 35

Optimal temperature of enzymes

Answer 35

37 degree Celsius

Question 36

Loss of the folding structure of a protein

Answer 36

Denaturation

Question 37

Inactivation of enzymes occurs at what temperature

Answer 37

60-65 deg.celsius

Question 38

Temperature of denaturation of enzymes

Answer 38

40-50 deg. Celsius

Question 39

Non-protein molecules necessary for enzyme activity

Answer 39

Cofactors

Question 40

Inhibitors binds to active site

Answer 40

Competitive inhibitors

Question 41

Inhibitors binds to other sites

Answer 41

Non-competitive inhibitor

Question 42

Inhibitors binds to ES complex

Answer 42

Uncompetitive inhibitors

Question 43

Substrate needed to achieve maximum velocity

Answer 43

Vmax

Question 44

Substrate needed to catalyze a reaction

Answer 44

Km

Question 45

Vmax remains but km increases

Answer 45

Competitive inhibition

Question 46

Vmax decreases but Km remains

Answer 46

Non-competitive inhibition

Question 47

Vmax and Km both decrease

Answer 47

Uncompetitive inhibition

Question 48

Breakdown of RBCs

Answer 48

Hemolysis

Question 49

Interference that increase the enzyme activity

Answer 49

Hemolyzed

Question 50

Interference that decreases the enzyme activity

Answer 50

Lipemic

Question 51

When or what phase do you measure your enzyme activity

Answer 51

Linear phase

Question 52

Measurement of enzyme activity that stops

Answer 52

Fixed

Question 53

Measurement of enzyme activity that has no ends or it is measured continuously

Answer 53

Continuous/kinetic

Question 54

What do you use to measure the enzyme activity continuously

Answer 54

Continuous - reading spectrophotometer